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Pezeshki A, Kadkhodamohammadi E, Mahmmodzadeh A, Haniloo A. Acanthamoeba spp. in Water Sources from Zanjan province, Northwestern Iran. J. Hum. Environ. Health Promot.. 2017; 2 (3)
URL: http://zums.ac.ir/jhehp/article-1-112-en.html

1- Assistant professor Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Student Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3- Professor Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4- Associate professor Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract:   (68 Views)
Background: The genus Acanthamoeba is an opportunistic protozoan parasite with worldwide distribution where it able to cause fatal granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK). This organism inhabits in the wide range of natural and man-made aquatic environments. There is no data about distribution of Acanthamoeba spp. in the water sources of Zanjan province. The present study was carried out to evaluate the presence of Acanthamoeba spp. in the various water resources of Zanjan province, northwest Iran, using both morphological and molecular methods.
Methods: Water samples were randomly collected from 30 water sources in different parts of Zanjan, Iran, between April 2015 and May 2016. Then, the samples were cultured on non-nutrient agar and the Acanthamoeba genus identified by morphological characters. Polymerase chain reaction (PCR) was performed using the 18S rRNA gene as a molecular marker.  
Results: The obtained data showed that, of the 60 water samples collected, 30 (50%) were positive for Acanthamoeba spp. according to morphological and molecular approaches.
Conclusion: The present investigation, the first report of the distribution of Acanthamoeba spp. in the various water sources of Zanjan province, gives baseline knowledge regarding water contamination with Acanthamoeba spp. in these areas and emphasizes the ……… 
 
Keywords: Acanthamoeba, Water, PCR, Zanjan, Iran
     
Type of Study: Research | Subject: Special
Received: 2017/08/23 | Accepted: 2017/09/5 | Published: 2017/09/5

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