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Mohammadi J, Mohammadi M, Mehrasbi M R, Peyda M. Investigation of Enzyme Immobilization Effects on its Characteristics. J. Hum. Environ. Health Promot.. 2016; 1 (4) :183-188
URL: http://zums.ac.ir/jhehp/article-1-55-en.html

1- MSc Department of Environmental Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan ,Iran
2- phD National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
3- phD Department of Environmental Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan ,Iran
Abstract:   (688 Views)

Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve their characteristics.

Methods; In this study silica coated magnetite nanoparticles were Functionalized with GPTSM as a linker, then immobilization reaction performed by using  various amounts of lipase B from Candida Antarctica (CALB), for the next step immobilization effects on thermal stability and optimum pH were investigated in comparison with free CALB.

Results; Results illustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55°C while free CALB loss its activity at the same condition.

Conclusion; Immobilization of CALB on Fe3O4@SiO2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free CALB.

Full-Text [PDF 524 kb]   (104 Downloads)    
Type of Study: Research | Subject: Special
Received: 2016/05/19 | Accepted: 2016/07/23 | Published: 2016/09/20

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