Volume 26, Issue 119 (November & December 2018)                   J Adv Med Biomed Res 2018, 26(119): 24-33 | Back to browse issues page

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Rezazadeh K, Chehelcheraghi F, Anbari K. The Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Survival of Random Skin Flap on Sterptozotocin-Induced Diabetic Rats. J Adv Med Biomed Res. 2018; 26 (119) :24-33
URL: http://zums.ac.ir/journal/article-1-5223-en.html
1- Student Research Committee, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Dept. of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran , chehelcheraghi.farzaneh@lums.ac.ir
3- Dept. of Social Medicine, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract:   (3086 Views)
Background & Objective:  Wound dressing and healing in diabetic patients is encountered with many problems. This study aims to investigate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the survival of random skin flap (RSF) on Streptozotocin-induced diabetic rats (STZ) using an optical microscope.
Materials & Methods:  In this study, 60 male Albino Wistar rats were used (average weight 250-300 gr). The rats were divided into six groups: 1). Health-Non (HN), 2). Health- Cells (HC), 3). Health –Sham (HS), 4). Diabetic-Non (DN) that were became diabetic by injecting STZ 70 mg/kg intraperitoneally), 5). Diabetic-Sham (DS), 6). And Diabetic-Cell (DC). In all groups, the day of surgery was considered as the zero day, on the back area of animal, the flap was created with a size of 8 × 3 cm and the BM-MSCs were performed. The sampling was performed on day 7 after surgery from the region where Transitional Zone (TZ) necrosis was initiated.
Results:  BM-MSCs increased the number of blood vessels (P=0.009) and the histology parameters (wound demarcation P=0.0001, granulation tissue P=0.0001) significantly compared to the control group. But this increase was not significant in the area of the survival region.
Conclusion:  It was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.
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✅ It was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.
Type of Study: Original | Subject: Medical laboratory and animal investigation
Received: 2018/06/13 | Accepted: 2019/08/18 | Published: 2018/10/1

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