Volume 23, Issue 97 (4-2015)                   zumsj 2015, 23(97): 72-81 | Back to browse issues page

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Mahmoudi M, Shahidi S, Golmohammadi H, Mohammadi S. The Effect of Echium amoenum Hydro-Alcoholic Extract on Blood Glucose level, Lipid Profile and Lipoproteins in Streptozotocin-induced Diabetic Male Rats. zumsj. 2015; 23 (97) :72-81
URL: http://zums.ac.ir/journal/article-1-3089-en.html
Abstract:   (7886 Views)

Background and Objective: Diabetes mellitus is one of the most important metabolic disorders worldwide. So far, numerous medicinal plants have been used to treat diabetes mellitus. Echium amoenum L. (EA) is one of the important medicinal herbs in Iranian traditional medicine. In the present study, the effects of EA hydroalcoholic extract on blood glucose, lipid profile and lipoproteins were studied in diabetic male rats. Materials and Methods: In this study, 30 male Wistar rats were randomly divided into five groups with six rats in each: normal control diabetic control and diabetic rats treated with hydroalcoholic extract of EA (100, 400 and 600 mg/kg). Diabetes was induced in the rats by administrating a single dose of streptozotocine (60 mg/kg, ip). Injection of extract was done intraperitoneally for 30 days. Finally, blood samples were taken and the concentration of glucose, triglyceride, cholesterol, HDL-C and LDL-C in the serum was measured. Results: Treatment of diabetic rats with EA extract (400 and 600 mg/kg) for 30 days significantly decreased glucose serum levels (P< 0.01). After EA extract administration, cholesterol, triglyceride and LDL-C levels decreased in all doses of EA extract in a dose dependent manner and this reduction reached statistical significance in the 400 and 600 mg/kg group in comparison to the diabetic control group (P< 0.01). Also, HDL level significantly increased compared to the diabetic control group (P< 0.01). Conclusion: This study indicates that hydroalcoholic extract of EA has hypoglycemic effect on diabetic rats and leads to valuable changes in blood lipid profiles as well as lipoprotein levels. References 1- Defronzo RA. Pathogenesis of diabetes. Diabetes Rev. 1997 5: 177. 2- Lasker SP, McLachlan CS, Wang L, Ali SMK, Jelinek HF. Discovery, treatment and management of diabetes. J Diabetology. 2010 14: 1-8. 3- Azizi F, Madjid M, Rahmani M, Emami H, Mirmiran P, Hadjipour R. Tehran lipid and glucose study (TLGS): rationale and design. Ir J Endo Metab. 2000 2: 77-86. 4- Chait A, Bierman E. Pathogenesis of macrovascular disease in diabetes, Joslin’s diabetes mellitus. Philadelphia. 1994. 5- Suji G, Sivakami S. Approaches to the treatement of diabetes mellitus: an overview. Cell Mol Biol. 2003 49: 635-39. 6- Murugesan N, Shobana R, Snehalatha C. Immediate impact of diabetes training programme for primary care physicians-an endeavour for national capacity building for diabetes management in India. Diab Res Clin Pract. 2009 83: 140-4. 7- Choi JS, Yokozawa T, Oura H. Improvement of hyperglycemia and hyperlipemia in streptozotocin diabetic rats by a methanolic extract of Prunus davidiana stems and its main component prunin. Planta Med. 1991 57: 208-11. 8- Ahmadi Mahmoudabadi N, Madani H,Mahzouni P. Hypoglycemic and hypolipidemic effect of hydroalcoholic Cynara scolymus L extract in alloxan monohydrate-induced diabetic rats to compare with glibenclamide. Ir J Med Aromatic Plants. 2008 24: 148-61. 9- Saiah Bargard M, Asadi M, Amini h, Saiah M, Akhondzadeh Sh, Kamali nejad, M. Efficacy of aqueous extract of Echium amoenum L In the treatment of mild to moderate major depressive disorder: a randomized double blind clinical trial. J Med Plants. 200410: 61-8. 10- Amirghofran Z, Azadbakht M, Keshavarzi F. Echium amoenum stimulate of lymphocyte proliferation and inhibit of humoral antibody synthesis. Ir J Med Science. 2000 25: 119-24. 11- Ranjbar A, Khorami S, Safarabadi M et al. Antioxidant Activity of Iranian Echium amoenum Fisch & C. Mey Flower Decoction in Humans: A cross-sectional Before/After Clinical Trial. Evid Bas Compl Alter Med. 2006 3(4): 469-473. 12- NRC (National Research Council), Institute of laboratory animal resources, commission on life sciences. Guide for the care and use of laboratory animals. 1996. 13- Mehrabani M, Mehrabani M, Raftari Sh, et al. Evaluation of hepatotoxicity of common doses of extract of Echium amoenum in rats. 2007 14: 44-54. 14- Kamboj A, Kumar S, Kumar V. Evaluation of Antidiabetic Activity of Hydroalcoholic Extract of Cestrum nocturnum Leaves in Streptozotocin-Induced Diabetic Rats.J Adv Pharmacol Sci. 2013 50: 401. 15- Freidwald WT, Levy R, Fredrikson D. Estimation of the concentration of LDL.c in plasma, without use of the preparative ultra centrifuge. Clin Chem. 1972 18: 499-502. 16- Naderi HM, Rezaee MB. Primory phytochemical investigation of Echium amoenum. Ir J Med Aroma Plants Res. 2004 20: 377-83. 17- Abolhassani M, Antiviral activity of borage (Echium amoenum). J Arch Med Sci. 2010 6(3): 366-369. 18- Handa SS, Chawla AS. Hypoglycemic plants. J Fitotrapia. 1989 60: 195-8. 19- Naga Raju GJ, Sarita P,Ramana GA, et al. Estimation of trace element in some anti-diabetic medicinal plants. Using PIXE technique Appl Radiat Isot. 2006 64: 893-900. 20- Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in strePtozocin-induced diabetic rats, Comp Biochem and Physiol C Toxicol Pharmacol J. 2003 135: 357-64. 21- Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocin-induced diabetic rats. Am J Physiol Endocrinol Metab. 2006 290: 339-46. 22- Chia C, Chena W, Chic T et al. Phosphatidylinositol-3-kinase is involved in the antihyperglycemic effect induced by resveratrol in streptozotocin-induced diabetic rats. Life Sci J. 2007 80: 1713-20. 23- Roghani M, Qeini M, Mousavi S, Ansari F, Sharayeli M. Evaluation of the effect of oral Amaranthus caudatus administration on reduction of blood glucose and lipids in female rat. Daneshvar Med J. 2010 17: 19-28. 24- Madani H, Ahmadi Mahmoodabadi N, Vahdati A. Effects of hydroalcoholic extract of Anethum graveolens on plasma glucose and lipid levels in diabetes induced rats. Ir J Diabt Lipid. 2006 2: 109-16. 25- Mehrabani M, Ghannadi A, Sajjadi E, Ghassemi N, Shams-Ardakani M.R. Toxic Pyrrolizidine alkaloids of Echium amoenum Fisch. & Mey. J DARU. 2006 14 (3): 122-7. 26- Sadeghzadeh F, Eidi A, Parivar K, Mazooji A. Hypoglycemic effect of alcoholic extract of Salvia nemorosa in normal and diabetic male rats. Pejouhesh J. 2008 32: 233-8. 27- Verspohl E.J. Recommended testing in diabetes research. Planta Med J. 2002 68: 581-90. 28- Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative effect of diasulin a polyherbal formulation in alloxan induced hyperglycemic rats.J BMC Compl Altern Med. 2005 5: 1–8. 29- Davalos A, Fernandez-Hernando C, Cerrato F, et al. Red grape juice polyphenols alter cholesterol homeostasis and increase LDL-receptor activity in human cells in vitro. J Nutr. 2006 136: 1766-73. 30- Shahrani M, Pilehvarian A, Khayri S, et al. Effects of Kelussia odoratissima Mozaffarian (KOM) extract on blood lipid in Balb/c mice. J Sharkord Univ Med Sci. 2009 10: 50-56 31- Gebhardt, R. Inhibition of cholesterol biosynthesis in primary cultured rat hepatocytes by artichoke Cynara scolymus L extracts. Pharmacol Experimenta J. 1998 286: 1122-28. 32- Sauvaire Y, Ribes G, Baccou JC, Loubatieeres-Mariani M. Implication of steroid saponins and sapogenins in the hypocholesterolemic effect of fenugreek. J Nutrition. 1991 26: 191-7. 33- Roghani M, Baluchnejadmojarad T, Roghani Dehlordi F. Hypolipidemic effect of aqueous leaf extract of Trigonella foenum-graecum in diabetic rats. Ir J Endocrinol Metab. 2005 7: 167-71. 34- Pal S, Ho N, Santo SC, et al. Red wine polyphenolics increase LDL receptor expression and activity and suppress the secretion of apo B100 from human hepG2 cells. J Nutrition. 2003 133: 100-6. 35- Borradaile N, Dreu L, Huff M. Inhibition of net hepG2 cell apolipoprotin B secretion by the citrus flavonoid narringenin involves activation of phosphatidylinositol 3-kinse, independent of insulin receptor substrate-1 phospho- rylation. J Diabt. 2003 52: 2554-61. 36- Arena MS. Harper's illustrated biochemistry, 27th ed Tehran: Taimourzadeh pub 2006 226-51. 37- Sayantani D, Dipak KB. Dietary effect of &gamma-Linolenic acid on the lipid profile of rat fed erucic acid rich oil. J Oleo Sci. 2007 56: 569-77. 38- Fragoso Y, Skinner E. The effect of gammalinolenic acid on the subfractions of plasma high density lipoprotein of the rabbit. Biochem Pharmacol J. 1992 44: 1085-90. 39- Ishikawa T, Fujiyama Y, Igarashi O, et al. Effects of gammalinolenic acid on plasma lipoproteins and apolipoproteins. J Atherosclerosis. 1989 75: 95-104.

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Type of Study: Applicable | Subject: General
Received: 2015/04/28 | Accepted: 2015/04/28 | Published: 2015/04/28

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