Volume 26, Issue 117 (9-2018)                   JAMBR 2018, 26(117): 32-43 | Back to browse issues page

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M I, Y M, M S, F S, S N, S H et al . Ex Vivo Expansion of Umbilical Cord Blood Hematopoietic Stem Cells on Collagen- Fibronectin Coated Electrospun Nano Scaffold . JAMBR. 2018; 26 (117) :32-43
URL: http://zums.ac.ir/journal/article-1-5404-en.html
Abstract:   (2234 Views)
Background and Objective: Umbilical Cord blood (UCB) hematopoietic stem cell (HSC) transplantation is a therapeutic approach for the treatment of malignant and non-malignant hematologic disorders due to ease of collection, lack of risk for donors and lower levels of infection. Moreover, it is considered a good alternative for bone marrow HSC transplantation. The main limitation of their use is insufficient amount of HSCs due to low volume of blood collected from umbilical cord. A possible solution to overcome this limitation may be the in vitro expansion of these cells on 3D nanofiber scaffolds, with the goal of natural niche’s topography and chemistry mimicking.
Materials and Methods: In this study, MACS isolated CD133+ cells were confirmed via flow cytometry and then cultured in three conditions:  2-dimensional culture (2D), 3D PLLA scaffold and collagen-fibronectin coated PLLA scaffold.
  • : Comparison between three aforementioned groups showed that collagen-fibronectin coated scaffold had the highest expansion level CD133+ cells, while also having the highest clonogenic capacity and biocompatibility.
Conclusion: The results of this study showed that the protein coating of 3D PLLA scaffold with collagen-fibronectin provides a suitable system for the expansion of cells with minimal differentiation in vitro.
Full-Text [PDF 3270 kb]   (698 Downloads)    
Type of Study: Original | Subject: General
Received: 2018/11/11 | Accepted: 2018/11/11 | Published: 2018/11/11

1. References
2. Wilson A, Trumpp A. Bone-marrow haematopoietic-stem-cell niches. Nat Rev Immunol. 2006; 6: 93-106.
3. Nagasawa T, Hirota S, Tachibana K, et al. Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the
4. CXC chemokine PBSF/SDF-1. Nature. 1996; 382: 635-8.
5. Mehta R, Rezvani K, Olson B, et al. Novel techniques for ex vivo expansion of cord blood: Clinical Trials. Front Med. 2015; 2: 89.
6. Mousavi SH, Abroun S, Soleimani M, Molwa SJ, et al. Expansion of human cord blood hematopoietic stem/progenitor cells inthree-dimensional Nanoscaffold coated with Fibronectin. Int J Hematol Oncol Stem Cell Res. 2015; 9: 72-79.
7. Eskandari F, Allahverdi A, Nasiri H, et al. Nanofiber expansion of umbilical cord blood hematopoietic stem cells. Iran J Pediat Hematol Oncol. 2015; 5: 170-78.
8. Bertolini F, Battaglia M, Lanza A, et al. Stem cell enumeration in cord blood vs bone marrow and peripheral blood. Bone Marrow Transplant. 1998; 1: S57.
9. Dravid G, Rao S. Ex vivo expansion of stem cells from umbilical cord blood: expression of cell adhesion molecules. Stem Cells. 2002; 20: 183-89.
10. Lu J, Aggarvval R, Pompili VJ, Das H. A novel technology for hematopoietic stem cell expansion using combination of nanofiber and growth factors. Recent Pat Nanotechnol. 2010; 4: 125-35.
11. Liu HC, Lee IC, Wang JH, Yang SH, Young TH. Preparation of plla membranes with different morphologies for culture of ligament cells. Biomatericals. 2004; 25: 4047-56.
12. Ma Z, C Gao, J Shen. Surface modification of poly-L-lactic acid (PLLA) membrane by grafting acrylamide: an effective way to improve cytocompatibility for chondrocytes. J Biomater Sci Polym Ed. 2003; 14: 13-25.
13. Islami M, Mortazavi Y, Soleimani M, Nadri S. In vitro expansion of CD 133+ cells derived from umbilical cord blood in poly-L-lactic acid(PLLA) scaffold coated with fibronectin and collagen. Artif Cells Nanomed Biotechnol. 2018;46: 1025-33.
14. Mokhtari S, Baptisat P, Vyas D, et al. A new approach to expand cord blood derived hematopoietic stem cells, using bioengineered human fetal liver tissue 3D-constructs. Blood. 2015. 126: 3097.
15. Mansourizadeh F, Asadi A, Oryan SH, Nematollahzadeh A, Dodel M, Asghavi M. PLLA/HA Nano composite scaffolds for stem cell proliferation and differentiation in tissue engineering. Molecular Biol Res Communicat. 2013; 2: 1-10.
16. Dubey G, Mequanint K. Conjugation of fibronectin onto three-dimensional porous scaffolds for vascular tissue engineering applications. Acta Biomaterialia. 2011; 7: 1114-25.
17. Colombo E, Calcaterra F, Cappelletti M, Marilio D, Dell Bella S, et al. Comparison of fibronectin and collagen in supporting the isolation and expansion of endothelial progenitor cells from human adult peripheral blood. PLoS One. 2013; 8: e66734.
18. Walasek MA, Van OSR, De Haan G. Hematopoietic stem cell expansion: challenges and opportunities. Ann N Y Acad Sci. 2012. 1266: 138-50.
19. Carletti E, Motta A, Migliaresi C. Scaffolds for tissue engineering and 3D cell culture. Methods Mol Biol. 2011. 695: 17-39.
20. Serebriiskii I, Castell-Cros R, Lamb A Golemis EA, Cukierman E, et al. Fibroblast-derived 3D matrix differentially regulates the growth and drug-responsiveness of human cancer cells. Matrix biol. 2008; 27: 573-85.
21. Celebi B, Mantovani D, Pineault N, Effects of extracellular matrix proteins on the growth of haematopoietic progenitor cells. Biomed Mater. 2011; 6: 055011.
22. Connor NS, Aubin JE, Sodek J, Independent expression of type I collagen and fibronectinby normal fibroblast-like cells. J Cell Sci. 1983; 63: 233-44.
23. Klamer S, Voermans C. The role of novel and known extracellular matrix and adhesion molecules in the homeostatic and regenerative bone marrow microenvironment. Cell Adh Migr. 2014; 8: 563-77.
24. Liu H, Lin J, Roy K. Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells. Biomaterials. 2006; 27: 5978-89.
25. Zheng Li, Zhen XU,Yingjun Liu, Wan R, Gao CH. Multifunctional non-woven fabrics of interfused graphene fibres. Nature Communicate. 2016; 7: 13684.
26. Chua KN, Char C, Lee PC, et al. Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials. 2006; 27: 6043-51.
27. Mc Carthy J, Turley EA. Effects of extracellular matrix components on cell locomotion. Crit Rev Oral Biol Med. 1993; 4: 619-37.
28. Hernandez T, Esparza M, Perez AB, et al. A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006; 79: 46-58.

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