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Showing 14 results for هاشمی

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Volume 10, Issue 38 (3-2002)

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Volume 11, Issue 43 (Jun 2003)

Volume 13, Issue 51 (Jun 2005)

Background & Objectives: The lateral nasal osteotomy is an integral part of cosmetic surgery (rhinoplasty). A reproducible and predictable technique with the least complication is a significant contributor to operation success. Regarding the different techniques and instrumentation for osteotomy, this study was carried out with the aim of comparison of ecchymosis severity due to lateral osteotomy in internal continuous and external perforated techniques in Tehran from 2004 to 2005. Materials & Methods: This clinical trial was conducted on 30 patients ( 15 males and 15 females ). The entire patients had the same situation pre and post operation. In each patient both internal and external osteotomy were performed , and ecchymosis severity due to operation was measured using a ruler on the 1st, 2nd, 3rd, 5th and 7th day following the operation. The data were analysed through SPSS (Smirnov & kolmograph, repeated measure and Wilks tests). Results: The study results indicated that the ecchymosis severity was far lower in external method than internal method (P=0.01)and it was higher in lower eyelids than in upper eyelids (P=0.01) . Ecchymosis severity was higher on the 3rd day than the other days and it gradually declined from 3rd to 7th days. Age had no significant association with ecchymosis severity while its rate was higher in men than in women (P=0.01). Conclusions: External osteotomy causes less ecchymosis severity compared to internal technique and has other advantages confirmed by other researchers. Thus, it can be introduced as a preferable educational technique.

Mh Esmaeili, K Parivar, P Yaghmaie, E Abbasi, H Jahani Hashemea,
Volume 14, Issue 55 (Jun 2006)

Background & Objectives: Reports suggest that co-administration of Matricaria Chamomilla (MC) extract with morphine greatly attenuates the dependence on morphine and its injection prior to naloxan inhibits the withdrawal syndrome. Locus Ceruleus (LC) and paragigantocellularis (PGi) nuclei play a key role in appearance of withdrawal syndrome. Thus, this study was conducted to determine the effects of MC extract injection into pGi nucleus on morphine withdrawal in rats. Materials & Methods:30 rats (Weighing 250-300gr) were divided into two groups of control (receiving saline) and morphine- treated. Following surgical implantation of cannula, morphine- treated group received morphine twice daily for 7 days. This group was classified into 4 sub-groups. The first sub-group received only morphine while the three remaining sub-groups were administed with Matricaria Chamomilla on day 7, five minutes prior to 1 microliter naloxan injection, with 10, 25, and 50 microgr/lit, respectively. In all groups 5 mg/kg naloxan was injected 3 hours after the final injection of morphine and withdrawal behavior (jumping and climbing) was investigated for 30 minutes. Results: The results showed that injection of all three high doses of MC extract particulary 25 microgr/microlit into PGi nuclens could significantly decline the symptoms of withdrawal syndrome. Conclusion: It seems that injection of MC extract into PGi nucleus could be beneficial to the treatrnent of morphine withdrawal syndrome in rats.

M Nasiri, M Jabbarpour, M Aminbakhsh, O Omrani, F Hashemi, M Heidarzade,
Volume 14, Issue 57 (Dec 2006)

Background & Objective: Down syndrome is one of the most common chromosome aneuploidies causing mental retardation which occurs in approximately 1/230 pregnancies. It is usually caused by the presence of an extra chromosome 21. The aim of this study was to evaluate the simple PCR based DNA diagnostic method and also to determine the parental origin of the extra chromosome 21 in trisomal Down syndrome. Materials & methods: To determine the polymorphism rates of chromosome 21 microsatellite markers, 50 people from Eastern Azarbayjan were randomly selected and studied for the microsatellites. The results were statistically analyzed. Thirty affected Down syndrome patients, diagnosed by specialists were referred to the lab for further molecular analysis. After genetic counseling and getting consent, blood samples were obtained. Seven pairs of chromosome 21 microsatellite markers were amplified using PCR in all the samples. Results: Five highly polymorphic microsatellite markers were selected from a total seven markers, studied in 50 normal people. Out of 30 Down syndrome’s patients, trisomal 21 was diagnosed in 21 families (70%). In which non-disjunction errors were determined to be of maternal origin in 86% and of paternal origin in 9% of the cases. The mean maternal and parental age was 33/3 and 36/2, respectively. Conclusion: The three microsatellite markers, D21S1910, D21S1411 & D21S11 could diagnose a high percentage of trisomal 21 in Down syndrome’ patients. The parental origin of an extra copy of chromosome 21 could be exactly determined.

Gh Saki, M Hashemitabar, B Ghavvamizadeh, Z Safikani, D Bijannejad, A Sobhani,
Volume 14, Issue 57 (Dec 2006)

The Evaluation of the Survival Rate and Motility of Freeze-Thawed Mouse Sperm -Frozen in and out of the Epididym. Saki GH, Hashemitabar M, Ghavvamizadeh B, Safikani Z, Bijannejad D, Sobhani A Corresponding Authoŕ s Address: Department of Anatomy, Tehran,University of Medical Science, Tehran, Iran. Email: Background & objectives: Direct contact between a sperm cell and cryopreservation solution has detrimental effects on the cell. In this study the role of the epididymal tissue in the preservation of direct contact between sperm cell and cryopreservation solution during a freeze–thaw process was studied by assessing motility and vitality of the sperm. Material & methods: About 30 male mice were killed and the right caudal epididymis were removed and placed in cryo-preservation solution for two minutes. The samples were exposed to liquid nitrogen vapor for ten minutes and then immersed in liquid nitrogen. The left caudal epididymis were similarly removed and placed in T6 medium. In order to extract sperm, samples were needled and incubated for 1 hour at 37ºC. Subsequently, sperm motility and vitality were assessed as control group and then the remaining solution was transmitted into a tube containing cryopreservation solution. For thawing, samples were picked up from a liquid nitrogen tank and kept in room temperature for 20 seconds and then immersed in warm water (37ºC) for 2 minutes. Thereafter, sperm motility and vitality were assessed. Results: The survival rates of the three groups (control, outer and inner epididym) were 78.75±13.01, 34.67±7.86 and 9.97±7.08, respectively. The statistical analysis has shown that the difference between the inner and outer epididym was significant (P<0.05). The progressive motility of sperms in the three groups was 30.29±15.33, 3.29±3.55 and 0.00±0.00, respectively. The progressive motility of sperms in the inner and outer epididym was less than the control group (P<0.05), but there was no significant difference between the inner and outer groups (P=0.344). The non-progressive motility of sperms in the three groups was 34.72±12.21, 29.21±10.37 and 6.78±4.94, respectively. The statistical analysis has shown that the difference between the control and outer groups and also between the control and inner groups was significant (P<0.05) but the difference between the inner and outer groups was not significant. Conclusion: The quality of cryopreserved–thawed sperm in the outer epididym group was significantly better than in the inner epididym group. In this study we can conclude that the epididym has no protective effect on sperm during cryopreserved processing.

M Hashemitabar, M Orazizadeh, Ls Khorsandi,
Volume 16, Issue 62 (3-2008)

Background and Objectives: Apoptosis (programmed cell death) is an important regulatory event in spermatogenesis. Abnormally accelerated apoptosis in germ cells, may lead to an imbalance between cell proliferation and death, resulting in impairment in spermatogenic. Some studies have shown that glucocorticoids affect testialar homeostasis by decreasing of testosterone level. In the present study, the influence of dexametasone (Dex), a widely used glucocorticoid agent, on expression of FasL (Fas-Ligand) protein (a proapoptotic protein) in mouse testicular germ cells is investigated. Materials and Methods: Twenty-four adult male (6- 8 weeks) mice were randomly divided into 3 groups. The first and second test groups received 2 and 7 mg/kg Dex per day, respectively, for 7 days. The control group received only saline daily for 7 days. One day after the final injection, the mice were sacrificed and the test groups were placed in formalin solution for immunohistochemistry studies. Positive immunoreactivity was calculated by H-score method. Results: The results revealed that expression of FasL in seminiferous epithelium is spermatogenic stage dependent, and the stage VII was the most susceptible to Dex. FasL expression was observed only at stages VII-VIII of spermatogenic cycle in 2 mg/kg Dex treated group (P<0.05). H-score was significantly increased in all stages of 7 mg/kg Dex treated group (P<0.05). The number of spermatocytes decreased significanhy in this group. Conclusions: It appears that glucocorticoid agents such as Dex, induces apoptosis by affecting proapoptotic proteins.

N Gheibi, H Jahani Hashemi, E Abbasi, Sm Hosseini, M Shahsavan,
Volume 16, Issue 63 (6-2008)

Background and Objective: Effects of electeromagnetic exposure on different parts of neruous system and memory of humans and animals has been established. In spite of important human studies, animal studies have been more precise and comprehensive. Extremely low frequency electromagnetic fields (ELF, <300Hz), have been reported to induce a variaty of behavioral and physiological function changes in animals. The object of present study was to determine the delay and durartion time of convulsions induced by co- exposure of ELF and strychnine. Materials and Methods: The effects of ELF on convulsions induced by strychnine (1mg/Kg) were investigated in 60 albino mice. Animals were devided into 6 groups (n=10), including control (I), 100Hz and 20Volt (II), 25Hz and 20Volt (III), 25Hz and 260Volt (IV), 100Hz and 260Volt (V), and 100Hz and 260Volt (VI). Delay time (Det) and duration time (Dut) of convulsions were measured respectively. Increase and decrease in Det and Dut were determined and were compared in control and five ELF exposed groups. Results: There was no significant difference in Det and Dut parameters between control (I) and III, IV and VI groups. In all these groups the convulsions terminated in animal death after a low Dut. In contrast there was a significant difference (P<0.001) in Det parameters between control (I) and the other groups (II and V). No deaths happened in groups (II and V). Conclusion: Co-exposure of higher ELF frequency and strychnine may decrease the epileptic effects of the drug.

Ls Khorsandi, S Bahramzadeh, M Hashemitabar, Sr Kalantar Mahdavi,
Volume 19, Issue 74 (3-2011)

Background and Objective: Metformin is a widely used medicine for treatment of type 2 diabetes. In this study, the effect of various doses of metformin on the mouse islets of langerhans volume was investigated. Materials and methods: Twenty four C57BL/6 adult male mice weighting 30±5 gr were randomly divided into 4 groups. Normal saline was given to the control group (group 4) and the experimental groups (groups 1-3) received 75, 150 and 300 mg/kg metformin daily by intraperitoneal injection for seven days. One day after the last injection the mice were sacrificed by cervical dislocation and their pancreases were fixed in 10% formalin for histological studies. The volume of the islets of langerhans was estimated by using Cavalieri method. Results: Volume of the islets of langerhans in doses of 75 and 150 mg/kg Metformin showed a non-significant difference in comparison to control group (P>0.05). 300 mg/kg metformin treated mice showed a significant increase in islets of langerhans volume compared to the control group (P<0.05). Conclusion: Metformin increases in the islets of langerhans volume in a dose-dependent manner. Increasing effects of Metformin on the islets of langerhans volume may be due to proliferation or hypertrophy of beta cells.

Z Hosseinpur, Sm Hashemi, E Salehi, T Ghazanfari,
Volume 21, Issue 89 (8-2013)

Background and Objective: Mesenchymal stem cells are promising sources of stem cells for tissue repair because of their ability to differentiate into different cells, easy proliferation and culture, and immunomodulatory properties. Despite extensive research on the immunophenotype of mesenchymal stem cells, a lack of specific markers comprises challenges for researchers. The aim of this research was to isolate and expand murine lung and adipose derived mesenchymal stem cells and to compare their expression of surface protein markers. Materials and Methods: Mesenchymal stem cells were isolated from murine lung and adipose tissues. Specific differentiation media were added for differential investigations. Finally, the expression of surface antigens were measured by flow cytometry and compared in both cell populations. Results: Mesenchymal stem cells from both tissues were proliferated well and adipocyte and osteocyte differentiations were observed. Moreover, they showed CD73, CD105 and CD90 expression, but no expression of CD34, CD45 and CD11b was observed. In this study, lung CD105+ mesenchymal stem cells possessed lower percentage than those in adipose tissue. Conclusion: Mesenchymal stem cells were isolated from lung similar to adipose derived mesenchymal stem cells and expressed common markers associated with adipose tissue mesenchymal stem cells.

F Mortazavi, Kh Abdolmaleki, Aa Samety, A Hashemian, Sh Haghjooy Javanmard,
Volume 22, Issue 94 (7-2014)

Background and Objective: Some evidence about the relationship between heme oxygenase and many cancers is available. Heme oxygenase has anti-apoptotic effects and contributes to tumor growth. The aim of this study was to evaluate the effect of heme oxygenase on melanoma tumor cells mitosis and tumor size in C57BL/6 mice. Materials and Methods: B16F10 melanoma cells were injected subcutaneously to 18 C57BL6 mice with 8 weeks of age. Mice were randomly divided into three groups: the first group received Zinc protoporphyrin (Znpp), heme oxygenase inhibitor (n = 6), the second group, hemin (heme oxygenase activator) (n = 6) and the third group((control group) (n = 6)) received diluent injection. They received their injection every other day from the first day of study for 16 days. Tumors were extracted in the 16th day of study. Length and width of tumors were measured and mitotic cell activities were evaluated using immunohistochemistry staining and counting Ki-67 posetive cells. Results: Our study results showed that size and weight of tumor and mitotic index in treated mice by Hemin were higher than two other groups. (P≤0.05). But mitotic index, tumor size and tumor weight in the recipient Znpp group and the control group were not statistically different. Conclusion: Stimulation of Hemeoxygenase-1 pathway by hemin injection can increase melanoma tumor growth. However, inhibition of this pathway by Hemeoxygenase -1 inhibitors may not be effective in reducing tumor growth and size. References 1- Elwood JM, Jopson J. Melanoma and sun exposure: an overview of published studies. Int J Cancer. 1997 73: 198-203. 2- Chang KW, Lee TC, Yeh WI, et al. Polymorphism in heme oxygenase-1 (HO-1) promoter is related to the risk of oral squamous cell carcinoma occurring on male areca chewers. Br J Cancer. 2004 91: 1551-5. 3- Halliwell B. Oxidative stress and cancer: have we moved forward? Biochem J. 2007 401: 1-11. 4- Brigelius-Flohe R, Flohe L. Basic principles and emerging concepts in the redox control of transcription factors. Antioxid Redox Signal. 2011 15: 2335-8. 5- Willett WC, MacMahon B. Diet and cancer--an overview. N Engl J Med. 1984 310: 633-8. 6- Maines MD. Heme oxygenase: function, multiplicity, regulatory mechanisms, and clinical applications. FASEB J. 1988 2: 2557-68. 7- Origassa CST, Câmara NOS. Cytoprotective role of heme oxygenase-1 and heme degradation derived end products in liver injury. World J of Hepatol. 2013 5: 541. 8- Doi K, Akaike T, Fujii S, et al. Induction of heme oxygenase-1 nitric oxide and ischaemia in experimental solid tumours and implications for tumour growth. Br J Cancer. 1999 80: 1945-54. 9- Maines MD, Abrahamsson PA. Expression of heme oxygenase-1 (HSP32) in human prostate: normal, hyperplastic, and tumor tissue distribution. Urology. 1996 47: 727-33. 10- Kiemer AK, Bildner N, Weber NC, Vollmar AM. Characterization of heme oxygenase 1 (heat shock protein 32) induction by atrial natriuretic peptide in human endothelial cells. Endocrinol. 2003 144: 802-12. 11- Henry F, Bretaudeau L, Barbieux I, Meflah K, Gregoire M. Induction of antigen presentation by macrophages after phagocytosis of tumour apoptotic cells. Res Immunol. 1998 149: 673-9. 12- Jozkowicz A, Was H, Dulak J. Heme oxygenase-1 in tumors: is it a false friend? Antioxidants & Redox Signaling. 2007 9: 2099-118. 13- Tiligada E. Chemotherapy: induction of stress responses. Endocr Relat Cancer. 2006 13 Suppl 1: S115-24. 14- Fang J, Sawa T, Akaike T, Greish K, Maeda H. Enhancement of chemotherapeutic response of tumor cells by a heme oxygenase inhibitor, pegylated zinc protoporphyrin. Int J Cancer. 2004 109: 1-8. 15- Yoshida C, Yoshida F, Sears DE, Hart SM, Ikebe D, Muto A, et al. Bcr-Abl signaling through the PI-3/S6 kinase pathway inhibits nuclear translocation of the transcription factor Bach2, which represses the antiapoptotic factor heme oxygenase-1. Blood. 2007 109: 1211-9. 16- Fang J, Sawa T, Akaike T, Greish K, Maeda H. Enhancement of chemotherapeutic response of tumor cells by a heme oxygenase inhibitor, pegylated zinc protoporphyrin. International Journal of Cancer. 2004 109: 1-8. 17- Mayerhofer M, Gleixner KV, Mayerhofer J, et al. Targeting of heat shock protein 32 (Hsp32)/heme oxygenase-1 (HO-1) in leukemic cells in chronic myeloid leukemia: a novel approach to overcome resistance against imatinib. Blood. 2008 111: 2200-10. 18- Mayerhofer M, Florian S, Krauth MT, et al. Identification of heme oxygenase-1 as a novel BCR/ABL-dependent survival factor in chronic myeloid leukemia. Cancer Res. 2004 64: 3148-54. 19- Nowis D, Legat M, Grzela T, et al. Heme oxygenase-1 protects tumor cells against photodynamic therapy-mediated cytotoxicity. Oncogene. 2006 25: 3365-74. 20- Fang J, Sawa T, Akaike T, et al. In vivo antitumor activity of pegylated zinc protoporphyrin: targeted inhibition of heme oxygenase in solid tumor. Cancer Res. 2003 63: 3567-74. 21- Hirai K, Sasahira T, Ohmori H, Fujii K, Kuniyasu H. Inhibition of heme oxygenase-1 by zinc protoporphyrin IX reduces tumor growth of LL/2 lung cancer in C57BL mice. Int J Cancer. 2007 120: 500-5. 22- Amjadi F, Javanmard SH, Zarkesh-Esfahani H, Khazaei M, Narimani M. Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production. J Exp Clin Cancer Res. 2011 30: 21. 23- Nowis D, Bugajski M, Winiarska M, et al. Zinc protoporphyrin IX, a heme oxygenase-1 inhibitor, demonstrates potent antitumor effects but is unable to potentiate antitumor effects of chemotherapeutics in mice. BMC Cancer. 2008 8: 197. 24- Devadas K, Dhawan S. Hemin activation ameliorates HIV-1 infection via heme oxygenase-1 induction. The Journal of Immunology. 2006 176: 4252-7. 25- Cesarovic N, Jirkof P, Rettich A, Nicholls F, Arras M. Combining sevoflurane anesthesia with fentanyl–midazolam or s-ketamine in laboratory mice. Journal of the American Association for Laboratory Animal Science JAALAS. 2012 51: 209. 26- Jensen MM, Jørgensen JT, Binderup T, Kjær A. Tumor volume in subcutaneous mouse xenografts measured by microCT is more accurate and reproducible than determined by 18F-FDG-microPET or external caliper. BMC Medical Imaging. 2008 8: 16. 27- Diehn F, Costouros N, Miller M, et al. Noninvasive fluorescent imaging reliably estimates biomass in vivo. Biotechniques. 2002 33: 1250-5. 28- Yang G, Nguyen X, Ou J, Rekulapelli P, Stevenson DK, Dennery PA. Unique effects of zinc protoporphyrin on HO-1 induction and apoptosis. Blood. 2001 97: 1306-13. 29- Murali R, Scolyer RA. Tumor-infiltrating lymphocytes and mitotic index in metastatic melanoma as predictors of patient survival. Proc Natl Acad Sci U S A. 2010 107(13): E46 author reply E7. 30- Motterlini R, Foresti R, Bassi R, Calabrese V, Clark JE, Green CJ. Endothelial heme oxygenase-1 induction by hypoxia. Modulation by inducible nitric-oxide synthase and S-nitrosothiols. J Biol Chem. 2000 275: 13613-20. 31- Shibahara S, Muller RM, Taguchi H. Transcriptional control of rat heme oxygenase by heat shock. J Biol Chem. 1987 262: 12889-92. 32- Lin F, Girotti AW. Hyperresistance of leukemia cells to photodynamic inactivation after long-term exposure to hemin. Cancer Res. 1996 56: 4636-43. 33- Gueron G, De Siervi A, Ferrando M, et al. Critical role of endogenous heme oxygenase 1 as a tuner of the invasive potential of prostate cancer cells. Mol Cancer Res. 2009 7: 1745-55. 34- Hill M, Pereira V, Chauveau C, et al. Heme oxygenase-1 inhibits rat and human breast cancer cell proliferation: mutual cross inhibition with indoleamine 2, 3-dioxygenase. The FASEB Journal. 2005 19: 1957-68. 35- Glanemann M, Schirmeier A, Lippert S, Langrehr J, Neuhaus P, Nussler A, editors. Cobalt-protoporphyrin induced heme oxygenase overexpression and its impact on liver regeneration. Transplantation proceedings. Elsevier 2005.

A Hashemi, F Fallah, A Taherpour, H Goudarzi, S Tarashi, S Erfanimanesh, M Roshani , Ms Amin ,
Volume 23, Issue 98 (5-2015)

Background and Objective: Klebsiella pneumoniae is one of the most important causative agents both in form of hospital acquired and community-acquired infections. This bacterium is one of the most prevalent pathogens that is isolated from hospitals and is associated with high mortality rates. The uprising trend of multi drug resistance among Klebsiella pneumoniae has limited the treatment options. The objective of this study was to detect Metallo-beta-Lactamases, Extended-spectrum beta-lactamases (ESBLs) and outer membrane porins among Klebsiella pneumoniae strains isolated from hospitalized patients in Taleghani and Mofid hospitals. Materials and Methods: This cross-sectional study was performed on 100 Klebsiella pneumoniae isolates from 2012 to 2013. Antibiotic susceptibility tests were performed via Kirby-Bauer disc diffusion and Broth Microdilution methods according to CLSI guidelines. Metallo-beta-Lactamases, Extended-spectrum beta-lactamases (ESBLs) producing strains were detected by phenotypic confirmatory test and Combination Disk Diffusion Test (CDDT), respectively. The blaAIM, blaSPM, blaDIM, blaIMP, blaVIM ، blaTEM، blaSHV and blaCTX-M genes were detected using PCR and sequencing method. The outer membrane porins including OmpK35 and OmpK36 were analysed throughSDS-PAGE, PCR and sequencing methods. Results: Out of 100 K. pneumoniae isolates, 48 (48%) and 3(3%) were ESBL and MBL positive, respectively. The presence of blaTEM-1, blaSHV and blaCTX-M-15 was detected in 24 (50%), 30(62.5%) and 28 (58.33%) isolates respectively, while other genes were not detected. Outer Membrane Porins, OmpK35, was detected among 30 (62.5%) of 48 ESBL-producing isolates while OmpK36 was found in 35(72.91%) of 48 ESBL-producing isolates. In this study, Fosfomycin and Tigecycline were more effective than other antibiotics. Conclusion: The high prevalence of ESBL-producing K. pneumoniae detected in this study is of great concern which requires infection control measures including antibacterial management and rapid detection of beta-lactamases-producing isolates. References 1- Amraei S, Eslami G, Taherpour A, Goudarzi H, Hashemi A. Detection of FOXMOX, and ACT genes in ESBL- producing Klebsiella Pneumoniae strains. J Mazandaran Univ Med Sci. 2014 24: 11-20. 2- Fallah F, Taherpour A, Vala MH, Hashemi A. Global spread of new delhi metalo-beta-lactamase-1 (NDM-1). Arch Clin Infect Dis. 2012 6: 171-7. 3- Fallah F, Vala MH, Goudarzi H, et al. Identification of extended-spectrum-beta-lactamases (ESBLs), metallo-beta-lactamases (MBLs), Amp-C and KPC ß-lactamases among Klebsiella pneumoniae isolated from adults and pediatric patients in Iran. Afr J Microbiol Res. 2013 7: 3254-61. 4- Fallah F, Borhan RS, Hashemi A. Detection of bla (IMP) and bla (VIM) metallo-beta-lactamases genes among Pseudomonas aeruginosa strains. Int J Burns Trauma. 2013 3: 122-4 5- Hakemi Vala M, Hallajzadeh M , Hashemi A , et al. Detection of ambler class A, B and D β-lactamases among Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolated from burn patients. Ann Burns Fire Disasters. 2014 27: 8-13. 6- Fallah F, Taherpour A, Borhan R, Hashemi A, Habibi M, Nia RS. Evaluation of Zataria MultiFlora Boiss and Carum copticum antibacterial activity on IMP-type metallo-beta-lactamase-producing Pseudomonas aeruginosa. Ann Burns Fire Disasters. 2013 26: 193. 7- Taherpour A, Hashemi A. Detection of OqxAB efflux pumps, OmpK35 and OmpK36 porins in ex-tended- spectrum-β- lactamase- producing Klebsiella pneumoniae isolates from Iran. Hippokratia. 2013 17: 355-8. 8- Hashemi A, Fallah F, Erfanimanesh S, Hamedani P, Alimehr S. Detection of β-lactamases and outer membrane porins among Klebsiella pneumoniae strains isolated in Iran. Scientifica. 2014 2014: 726179. 9- Tsai Y-K, Fung C-P, Lin J-C, et al. Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence. Antimicrob Agents Chemother. 2011 55: 1485-93. 10- Landman D, Bratu S, Quale J. Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae. J Med Microbiol. 2009 58: 1303-8. 11- Hashemi A, Shams S, Kalantar D, Taherpour A, Barati M. Antibacterial effect of Methanolic extract of Camellia Sinensis L. on Pseudomonas aeruginosa strains producing β-lactamases. J Gorgan Uni Med Sci. 2012 14: 136-42. 12- Hashemi A, Shams S, Barati M, Samedani A. Antibacterial effects of methanolic extracts of Zataria multiflora, Myrtus communis and Peganum harmala on Pseudomonas aeruginosa producing ESBL. Arak Med Univ J. 2011 14: 104-12. 13- Shakibaei M, Shah CF, Hashemi A, ADELI N. Detection of TEM, SHV and PER type extended-spectrum β-lactamase gene among clinical strains of Pseudomonas aeruginosa isolated from burnt patients at Sbafa-Hospital, Kerman, Iran. Iran J Basic Med Sci. 2008 2: 104-111. 14- Jafari M, Fallah F, Borhan RS, et al. The first report of CMY, aac (6′)-Ib and 16S rRNA methylase genes among Pseudomonas aeruginosa isolates from Iran. Arch Pediatr Infect Dis. 2013 2: 109-12. 15- Vala MH, Nowroozi J, Ghazi F, Tabatabai PN, Haghighi S. Comparing invasive and non-invasive of isolated Shigella flexneri by electron microscopy of cell culture, SDS-Page and congo red method. Iran Biomed J. 2007 11: 47-52. 16- Mansouri S, Kalantar D, Asadollahi P, Taherikalani M, Emaneini M. Characterization of Klebsiella pneumoniae strains producing extended spectrum beta-lactamases and AMPC type beta-lactamases isolated from hospitalized patients in Kerman, Iran. Roum Arch Microbiol Immunol. 2012 71: 81-6. 17- Ghafourian S, Bin Sekawi Z, Sadeghifard N, et al. The prevalence of ESBLs producing Klebsiella pneumoniae isolates in some major hospitals, Iran. Open Microbiol J. 2011 5: 91-5. 18- Feizabadi MM, Delfani S, Raji N, et al. Distribution of bla TEM, bla SHV, bla CTX-M genes among clinical isolates of Klebsiella pneumoniae at Labbafinejad hospital, Tehran, Iran. Microb Drug Resist. 2010 16: 53-49. 19- Goudarzi H, Aghamohammad S, Hashemi A, Nikmanesh B, Noori M. Distribution of blaTEM, blaSHV and bla CTX-M genes among Escherichia coli isolates causing urinary tract infection in children. Arch Clin Infect Dis. 2013 8: e1b207. 20- Keramati N, Zeighami H, Haghi F. Frequency of class I and II integrons in metalobetalactamase producing clinical isolates of Pseudomonas Aeruginosa. J Zanjan Univ Med Sci. 2014 22: 111-9. 21- Hemmati F, Soroori Zanjani R, Haghi F, Zeighami H. Determination of Antibiotic Resistance Profile and Frequency of Metallo-Beta- Lactamases in Pseudomonas Aeruginosa Isolates. J Zanjan Univ Med Sci. 2014 22: 77-85. 22- Fallah F, Noori M, Hashemi A, et al. Prevalence of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM genes among Acinetobacter baumannii isolated from two hospitals of Tehran, Iran. Scientifica. 2014 2014: 245162. 23- Hernández-Allés S, del Carmen Conejo M, Pascual A, Tomás JM, Benedí VJ, Martínez-Martínez L. Relationship between outer membrane alterations and susceptibility to antimicrobial agents in isogenic strains of Klebsiella pneumoniae. J Antimicrob Chemother. 2000 46: 273-7.

S Tarashi, H Goudarzi, A Hashemi, N Yousefi Nojokambri, M Sadreddin Amin, E Taki, ,
Volume 25, Issue 108 (3-2017)

Background and Objective: Efflux pumps are one of the major resistance mechanisms in Pseudomonas aeruginosa that inhibit the accumulation of antibiotics in bacterial cells. The aim of this study was determining the effect of carbonyl cyanide 3-chlorophenyl hydrazone (CCCP) and detecting the overexpression of pump MexCD-oprJ in P. aeruginosa isolated from burn patients in Shahid Motahari hospital during 2014 and 2015.

Materials and Methods: This descriptive study was performed on 100 P. aeruginosa isolates from burn patients in Shahid Motahari hospital. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and broth microdilution methods according to the CLSI guidelines. The effect of CCCP was determined using the broth microdilution method. The expression levels of MexD-oprJ (an efflux pump) was evaluated by Real-Time PCR and finally detection of nfxB mutations was carried out by PCR and sequencing techniques. 

Results: Antibiogram for 100 P. aeruginosa showed maximum susceptibility and resistance to colistin (100%) and ticarcilin (98%), respectively. The inhibitory effect of CCCP was apparently seen in 16 isolates. Real-time PCR results for these strains showed overexpression in range of 0.26-15.34 (mean: 4/1). The PCR and sequencing techniques showed mutations that converts lucin to prolin in position 14 of the protein sequence.

Conclusion: The increase in expression level of efflux pump MexCD-oprJ shows the prevalence of nfxB mutants in burn wound infections. Therefore, use of carbonyl cyanide 3-chlorophenyl hydrazone (CCCP) can help disrupt the function of efflux pumps and improve the effects of antibiotics.

, , , ,
Volume 26, Issue 114 (3-2018)

Background and Objective: Metabolic syndrome encompasses a series of metabolic disorders associated with increased cardiovascular risk. Currently, some studies are performed on the effects of physical activity as an alternative treatment for metabolic syndrome. To this end, the present study was set to assess the probable effects of continuous and interval aerobic trainings on metabolic syndrome among the elderly men.
Materials and methods: 60 eligible elderly men were selected and assigned to three groups of:  continuous training, interval training and the control. The initial blood samples were taken to record cardiovascular risk factors. After 48 hours, the subjects in the experimental group performed pre-designed training for 6 weeks and 3 sessions per week and the control group continued their daily activities. At the end of the training, further blood samples were taken again. The data were analyzed using one-way ANOVA, Scheffe post hoc and dependent t-test.
Results: Findings of this study revealed that after six weeks of continuous aerobic exercise training, systolic blood pressure, blood sugar and triglycerides significantly decreased. However, waist circumference and HDL did not show any significant change. The findings of this study showed significant changes in blood sugar, triglycerides and HDL in the interval training group. However, blood pressure and waist circumference did not indicate any significant changes.
Conclusion: According to the findings of this research, it can be asserted that both methods of aerobic training positively affect blood glucose and triglyceride signifying that interval aerobic exercise plays a major role in   increasing HDL, while continuous aerobic exercise reduces systolic blood pressure.

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