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Showing 28 results for بیضه

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Volume 4, Issue 15 (6-1996)
Abstract


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Volume 5, Issue 18 (3-1997)
Abstract


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Volume 8, Issue 33 (12-2000)
Abstract


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Volume 11, Issue 42 (3-2003)
Abstract


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Volume 11, Issue 44 (9-2003)
Abstract


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Volume 12, Issue 46 (3-2004)
Abstract


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Volume 13, Issue 50 (3-2005)
Abstract

Background and Objectives: One of the complications arising from the consumption of Cuminum Cyminum is reproductive system disorders in humans and animals. Since there has been no comprehensive research on the effect of this herbal medicine on spermatogenesis and production of testosterone in males, the present study was carried out with the aim of determining the effect of Cuminum Cyminum alcoholic extract on spermatogensis and testosterone production in adult male wistar rats in Kazeroon in 2004. Materials and Methods: In this experimental research 50 male wistar rats were divided into 5 groups of 10. Following the bioassay LD50 (lethal dose) of the compound was determined to be 120 mg/kg and concentrations of 30,60 and 100 mg/kg as sublethal doses were injected into peritoneum every other day for 21 days in three treatment groups. The two remaining groups received the solvent (distilled water) and no compound. The results were analysed through one-way analysis of variance and t-test. Results: The results showed that 100 mg/kg of Cuminum Cyminum alcoholic extract reduced serum testostrone level by 71.5% (P=0.05), while it had no significant effect on serum FSH and LH levels. Histological investigations of testis showed a decline of 19.7%, 48.3% and 89.5% sperm cells in doses 30, 60 and 100 mg/kg respectively. However, no adverse effect was observed owing to the injection. Conclusion: According to the research results it can be stated that Cuminum Cyminum alcoholic extract at 100 mg/kg of rats has probably caused the weakened function and reduced testosterone level and spermatogenesis through direct effect on testicular tissues. Research concerning the ultra structure studies of testis and the effect of the plant on hormone level in hypothalamus and fetus growth is recommended.


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Volume 13, Issue 53 (12-2005)
Abstract


M Aghdasi, Sh Oryan, K Parivar,
Volume 15, Issue 58 (3-2007)
Abstract

Background & objective: With regard to increasing use of opioids and their potential role in infertility research centers around the world are in search of pharmacologic compounds which could neutralize effects of opioids and overcome infertility through administration of GnRH and its analogues which also do not have considerable side effects. Materials & Methods: In this study male wistar rats weighing 200-250 gr were used. At different intervals (5-10-15 days) 5mg/kg morphine was injected intraperitonneally into the male rats. Then they were divided in 5 groups of 8. The first group contained intact rats, while saline, morphine, naloxone, and fertagyl were injected into the second third, fourth and fifth groups respectively. Then the rats were anesthetized and their bloods were taken for further tests. Results: The results showed that morphine induces loss of testis weight and diameter, loss of weight in rats, and nutritional and behavior changes. Furthermore, a significant changes in the amounts of LH and testosterone hormones was observed in all groups (p<0.05) while no significant change in the amount of FSH was observed. Conclusion: Since the experimental groups into which naloxone and GnRH were injected showed no significant difference, we suggest that naloxone and GnRH be administrated as opioid antagonist to slove the problem of morphine-induced infertility.


M Shariati, E Sharifi, M Kaveh,
Volume 15, Issue 61 (12-2007)
Abstract

Background and Objective: The pit of Phoenix dactylifera contains different chemical compounds such as saturated and unsaturated fatty acids, Zinc(Zn), Cadmium(Cd), Calcium(Ca), and potassium(K). Saturated fatty acids include stearic and palmitic acid and unsaturated fatty acids contain linoleic and oleic acids which could inhibit 5- - reductase enzyme. The present study was carried out with the aim of determining the effect of phoenix dactylifera pit powder on spermatogenesis and testosterone level in adult male rats.

 Materials and Methods: In this experimental research 45 wistar male rats were divided into 5 groups of 9 including the control group receiving no treatment, the saline group receiving an equal volume of normal saline as a solvent and the treatment groups receiving 0.05, 0.1 and 0.2 mg/kg body weight of Phoenix dactylifera pit powder orally for 21 days. The results were analysed through Excell, One-way analysis of variance and t-test.

 Results: The results showed that 0.1 and 0.2 mg/kg body weight of the powder increased the testosterone level significantly compared to the control and saline groups (P<0.05), whereas no significant change was observed in serum FSH and LH levels. Likewise, the above- mentioned amounts reduced dihydrotestosterone level in treatment groups (P<0.05). Histologic exam of the testis showed an increase in sperm density in seminiferous tubules of treatment groups (P<0.05).

 Conclusion: According to the research results it can be stated that the powder of phoenix dactylifera pit has probably caused increased testosterone level and decreased dihydrotestosterone level via inhibiting 5- - reductase enzyme induced by palmitic, stearic, linoleic, and oleic acids.


M Hashemitabar, M Orazizadeh, Ls Khorsandi,
Volume 16, Issue 62 (3-2008)
Abstract

Background and Objectives: Apoptosis (programmed cell death) is an important regulatory event in spermatogenesis. Abnormally accelerated apoptosis in germ cells, may lead to an imbalance between cell proliferation and death, resulting in impairment in spermatogenic. Some studies have shown that glucocorticoids affect testialar homeostasis by decreasing of testosterone level. In the present study, the influence of dexametasone (Dex), a widely used glucocorticoid agent, on expression of FasL (Fas-Ligand) protein (a proapoptotic protein) in mouse testicular germ cells is investigated. Materials and Methods: Twenty-four adult male (6- 8 weeks) mice were randomly divided into 3 groups. The first and second test groups received 2 and 7 mg/kg Dex per day, respectively, for 7 days. The control group received only saline daily for 7 days. One day after the final injection, the mice were sacrificed and the test groups were placed in formalin solution for immunohistochemistry studies. Positive immunoreactivity was calculated by H-score method. Results: The results revealed that expression of FasL in seminiferous epithelium is spermatogenic stage dependent, and the stage VII was the most susceptible to Dex. FasL expression was observed only at stages VII-VIII of spermatogenic cycle in 2 mg/kg Dex treated group (P<0.05). H-score was significantly increased in all stages of 7 mg/kg Dex treated group (P<0.05). The number of spermatocytes decreased significanhy in this group. Conclusions: It appears that glucocorticoid agents such as Dex, induces apoptosis by affecting proapoptotic proteins.


M Modaresi, M Messripour, M Asadi Marghmaleki, Mk Hamadanian,
Volume 16, Issue 63 (6-2008)
Abstract

Background and Objective: Saffron is widely used as a food flavor and has well known medicinal effects. Recent studies have revealed that main components of saffron including carotenoids: crocin, crocetin, picrocrocin and safranal have a large number of physiological effects on different biological systems. We decided to assess the possible effect of saffron extract on concentratians of LH, FSH and testosterone in mice. Materials and Methods: Four groups each including eight adult male Balb/C mice weighing 30 5g were chosen. Normal saline was administered as placebo to control group and saffron extract in doses of 25 , 50 and 100 was injected intraperitoneally for 20 days to test groups. Serum FSH, LH and testosterone, were measured using ELISA. Results: The level of FSH, LH and testosterone significantly increased in 100 saffron treated group, as compared with with placebo group. No significant differences were observed between other test groups and placebo. Conclusion: The study indicates efficacy of saffron extract in dose of 100 mg/kg/ 48h on pituitary-testis axis in mice. However further studies are needed to determine the effect of saffron on human reproductivity.


R Parandin, Hr Sadeghipour Rodsari, S Shamili, Hr Ghasempour,
Volume 16, Issue 65 (12-2008)
Abstract

Background and Objective: Evaluation of the importance and the effects of plant derived drugs on fertility of laboratory animals have long been recognized. In this research the effects of administration of aqueous extract of Boswellia thurifera on fertility indices, body weight and reproductive organs weight was evaluated in male rats. Materials and Methods: Eighteen rats were randomly divided into 3 groups, control, group A and group B. Each group comprises of six rats. Animals in control group received 1 ml of distilled water (vehicle) and test groups (A and B) received graded doses of 200 and 400 mg/kg body weight of aqueous extract of Boswellia thurifera respectively on daily basis for 60 days. At the end of 60 days of treatment period, fertility indices such as body weight, reproductive organs weight, sperm motility and count, fertility rate, epididymal sperm reserve (ESR), daily sperm production (DSP) and blood testosterone concentration were measured. Results: There was a significant increase in the reproductive organs and body weight, sperm motility and count, ESR, DSP, blood testosterone concentration and fertility rate especially in the group which received the higher doses of aqueous extract of Boswellia thurifera. Conclusion: The results of this study showed that aqueous extract of Boswellia thurifera could increase fertility rate in male rats. Key words: Boswellia thurifera, Fertility, Male rats


F Rezaeian, F Zamene Milani, Sf Miryousefi, A Jafari, A Kazemi, J Mohtadinia, R Mahdavi, M Vahed Jabbari, Sj Ghaem Maghami, Ar Ostad Rahimi,
Volume 17, Issue 68 (9-2009)
Abstract

Background and Objectives: Contamination of storage wheat to zearalenone producer molds represents a significant hazard to consumer health and has thus received increasing attention from food safety authorities and legislators. Various species of Fusarium genus are producer of different mycotoxins including zearalenone at certain conditions. These mycotoxins can enter into the human food and animal feed chain and cause premature or early thelarche, gynecomastia, primary and secondary mycotoxicosis in humans and animals. Materials and Methods: In this survey, contamination of 162 randomly collected wheat samples to zearalenone producer Fusarium was investigated using direct microscopic examination and culturing samples in specific media. Results: From studied 162 collected wheat samples, 160 (99%) samples had fungal contamination. Aspergillus niger, Penicillium Sp, other species of Asergillus (A. fumigatus, A. flavous, A. niveus, A. cchraceus,…) Fusarium Sp, Alternaria Sp, Cladesporium Sp, Gliocladium Sp. and Trichotecium Sp. were the most frequently detected microorganisms respectively. Conclusion: The presence of moulds such as Aspergillus Sp, Penicillium Sp, Fusarium Sp, Alternaria Sp, Cladesporium Sp, Gliocladium Sp, and Trichotecium Sp. in consumer wheat samples can lead to the presence of harmful mycotoxins and then could result in serious toxicity and illness in humans. As the fungal contamination has a strong influence on the ultimate quality of wheat, wheat end products and food safety, more attention to the quality and safety of wheat and wheat stored places are necessary. Key words: Wheat, Fungal contamination, Mycotoxins, Zearalenone, East Azarbaidjan


F Morteza Zadeh , Mm Taghavi, M Movahhedein, Hr Jafari Naveh,
Volume 18, Issue 72 (11-2010)
Abstract

Background and Objective: Efforts to develop new methods for a safe male contraceptive have been made during recent decades, but the most reliable method is still sterilization by vasectomy. In this experimental study, effects of administration of testosterone in combination with ultrasound on contraceptive male mice were studied. Materials and Methods: 28 mature male mice were randomly divided into the following groups: normal group, testosterone group, ultrasound group and testosterone-ultrasound group. Animals were injected with or exposed to normal saline, testosterone enanthate, ultrasound and testosterone enanthate in combination with ultrasound respectively for 6 weeks. After this period, viability, motility rate and number of sperms were assessed. The morphometric parameters such as tubular diameter were also studied. Results: In the testosterone group, the number of sperms decreased significantly in comparison to the 1st and the 3rd groups (P ≤0.01). The percentage of sperm viability was significantly decreased in the testosterone group comparing to other two groups (P ≤0.001). In concern to the percentage of sperm motility, a significance difference was not observed. The diameter of seminiferous tubules and the number of spermatogonia were changed in ultrasound group. In this group, the compacted seminiferous tubules were seen, so the number of counted cells was increased. In the testosterone group, the epithelium height decreased significantly. Conclusion: It seems that the ultrasound did not have effect on the absorption of testosterone and the histological changes resulting from ultrasound may be mechanical and thermal.


Z Ahmadi, M Ramezani, D Sohrabi,
Volume 19, Issue 74 (3-2011)
Abstract

Background and Objective: Cefixime is an antimicrobial agent which has a widespread ability against various pathogens, especially gram-negative organisms. Today, physicians apply cephalosporins especially cefixime in a wide scale. Regarding the side effects of some of these antibiotics on reproductive system, this study was conducted to determine the effect of cefixime on pituitary- gonadal hormones, gonadotrophins and testes morphology in adult male mice. Materials and Methods: Eighteen male mice (age: 12-16 weeks, weight: 35 5 gr) were divided into three groups control, sham and experimental (6 mice in each group). Experimental group received cefixime (0.5 gr/kg/day) as a solution in dimethyl solfoxide (DMSO) for 10 days the sham group received only drug solvent (DMSO) via IP injection and the control group remained intact. The animals were weighed and sacrificed. Level of hormones was measured by Radioi Immuno Assay (RIA) method. Then, tissues were fixed in Buin's fixative. Sections were cut into 5 µm thicknesses and stained with Hematoxylin and Eosin (H& E). Data were analyzed using T-test and SPSS software. Results: Count of spermatogenic, Sertoli and leydig cells and titer of FSH significantly decreased in the experimental group in comparison with the control and sham groups (P<0.01 and P<0.05). In the experimental group, DHEA hormone decreased significantly (P<0.05) in comparison with sham. No significant differences were seen in other factors between the groups. Conclusion: Regarding physiological role of Sertoli cells during spermatogenesis, reduction of FSH hormone may lead to negative effects on the sperm production and reproductive potential of male mice.


Ar ‌vahidi, Ma Khalili, O Amini-Rad,
Volume 19, Issue 76 (5-2011)
Abstract

Background and objective: One of the causes of male infertility is the lack of proper mobility. One way to deal with this problem is to add adenosine to human sperms in the laboratory. Therefore, this study aimed to determine the effect of different doses of adenosine on sperm motility in the ejaculates of fertile and infertile individuals. Materials and Methods: In this experimental laboratory study, 60 samples of seminal fluid of infertile and fertile men who referred to Yazd Infertility Center were analyzed according to WHO guidelines. Sperm counts, morphology and motility (fast, slow and immotile) were studied. After using Swim-up technique, the washed samples were divided into 4 groups, containing doses of 0, 2, 5 and 10 mg adenosine. The sperm parameters were evaluated after the addition of adenosine. Data were analyzed statistically using paired T-test and independent T-test. Results: The initial assessment indicated significant differences in sperm parameters, except for slow motility between the two groups. In fertile group, dose of 5 mg adenosine increased sperm count, and rapid motility, but lowered the rates of sperm immotility at doses of 5 and 10 mg. In the infertile group, sperm count improved at dose of 5 mg adenosine, but it showed no effect on slow sperm motility. 5 mg adenosine also improved rapid sperm motility, but dose of 10 mg had no effect. Both 5 and 10 mg adenosine significantly lowered rates of immotile sperms. Conclusion: Based on the results of this study, it can be concluded that addition of 5 mg adenosine to washed sperms can improve sperm motility of infertile men in vitro.


M Fakhraei, V Nejaty, N Dalirazh,
Volume 20, Issue 78 (3-2012)
Abstract

Background and Objective: Compounds of heavy metals such as vanadium, nickel, and cobalt can be useful in treatment of many diseases. There are several reports on the biological effects of vanadium compounds including insulin-like action and reduction of hypertension. A number of studies on vanadium have shown its promising ability to inhibit cancers in a variety of malignant cell lines. The goal of this study was to examine the anti-proliferative and apoptosis-inducing characteristics of some newly synthesized shifft-based vanadium (VOLComplex:C19H20N2O5V) on K562 leukemia cells. Materials and Methods: The MTT results show that the K562 cells viability is sensitive to the Vol complex in a concentration dependent manner. It was evident that the VOL complexe, up to a dose of 350 µg/ml, were non-cytotoxic in vitro after 48 hours of incubation time. In order to investigate if the Vol complexe have just anticancer effect, we designed further studies with non-cytotoxic doses of the complex. Based on the cell viability data, the concentrations of 150, 250, and 350 µg/ml of the VOL complexe were selected to treat the K562 cells for 12, 24, and 48 hr to induce apoptosis. Results: The results of apoptosis analysis and flow cytometric examination show that exposure of the K562 cells to non-cytotoxic dosses of the VOL complex leads to induction of apoptosis in a dose- and time-dependent manner. The highest level of apoptosis (37.96%) occurred after 48 hr of treatment in response to 350 µg/ml of the Vol complex. Moreover, the cell cycle analysis shows that the VOL complex induces a G0/G1 arrest. Conclusion: Our findings indicate that the Vol complex, even at a non-cytotoxic dose, has an apoptosis inducing effect, and that it is capable of arresting the affected cells in the G0/G1 phase of the cell cycle. Taken together, based on their role on induction of apoptosis and cell cycle arrest, VOL complexes may have the potential for being included in an anti-cancer drug discovery program in the near future.


R Eghdam Zamiri, M Moghimi, N Mosavi Nasab, H Amirmoghadami, M Joghatae , A Feizi,
Volume 20, Issue 81 (9-2012)
Abstract

Background and Objective: Elevation of the b-HCG serum levels has been reported in several tumors including breast cancer, and it is usually associated with aggressiveness. The aim of this study was to examine the possible correlation between the b-HCG serum levels and different grades of breast cancer tumors in patients undergoing chemotherapy. Materials and Methods: This cross-sectional study was conducted in the city of Zanjan during 2009-10. Serum samples from 56 cases of breast cancer patients were collected after surgery and prior to chemotherapy for analysis of total free b-HCG by electro chemiluminescence immunoassay, and the same procedure was repeated after 8 courses of chemotherapy. The b-HCG serum levels were compared in poor versus mild to moderate grades before and after chemotherapy. Results: In 37 cases of mild to moderate grade tumors, the mean b-HCG level was 1.09 ±1.4 miu/ml compared with 1.2 ±0.3 miu/ml (P= 0.75) in 29 cases of poor grade tumors. The mean b-HCG levels before and after chemotherapy were 1.15 ±1.4 miu/ml and 1.17 ±1.4 miu/ml (P=0.24), respectively. Conclusion: We did not find any significant association between the b-HCG serum levels and breast cancer tumor grades. Furthermore, chemotherapy does not appear to have an effect on b-HCG serum levels.


M Sofian, F Zolfaghari, H Sarmadian, A Ramezani, Aa Farazi,
Volume 20, Issue 82 (7-2012)
Abstract

Background and Objective: Brucellosis is an enzootic disease that can involve many organs and tissues. Brucella epididymo-orchitis is a focal complication of the brucellosis. The aim of this study was to compare epidemiologic, clinical, and laboratory features of patients suffering from Brucella induced epididymo-orchitis with cases of nonspecific epididymo-orchitis. Materials and Methods: This analytical cross-sectional study was performed in Valiasr Hospital in Arak from 2007 to 2011. A total of 40 cases of Brucella epididymo-orchitis were compared with 40 cases of bacterial (non-specific) epididymo-orchitis and the data were analyzed by SPSS ver.16 software and Student t-test, and Mann-Whitney U and chi-square tests. Results: We found no significant differences between the age (P-value = 0.82), fever (P-value = 0.17), history of urologic manipulation (P-value= 0.23), increased ESR (P-value = 0.28), and positive CRP (P-value = 0.45) between the two groups. However, there was a significant difference between the presence of arthritis and arthralgias (P-value = 0.02), leukocytosis (P-value <0.05), pyuria (P-value = 0.002), symptoms of dysury-frequency (P-value = 0.004), sweating (P-value <0.05), and location (P-value =0.004) between the two groups. Conclusion: This study shows that the existence of epididymo-orchitis without symptoms like dysuria and frequency, leukocytosis, and pyuria is suggestive of brucella epididymoorchitis. The physicians in endemic areas could use these findings for expediting the diagnosis and treatment of Brucella epididymo-orchitis.



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