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Showing 8 results for Feizi

M Sadeghi Shabestari, H Hosseinpour Feizi,
Volume 14, Issue 54 (Mar 2006)

Adverse reactions induced by BCG vaccination are rare and appear either in the form of lymphadenitis or osteitis. One of the rarest complications of the vaccine is disseminated mycobacterial infection which mostly occurs in infants with immune deficiency. In this paper a case of disseminated BCG infection is reported in a four-month-old infant suffering from severe combined immunodeficiency (SCID). Plain radiographs showed multiple osteolytic lesions in skull and extremities abdominal sonography revealed multiple nodules in the liver and spleen in thoracic radiography disseminated interstitial infiltration was observed, and gastric tub age culture tested positive for Mycobacterium bovis.

H Khani, Ma HosseinpoureFeizi, N Pouladi, N Chaparzadeh, V Montazeri, P Azarfam,
Volume 20, Issue 78 (3-2012)

Background and Objectives: Breast cancer (BC) is the most common invasive malignancy affecting women worldwide. The tumor-suppressor P53 gene (P53) is frequently mutated in breast tumors. To use P53 as a target for therapy, it is important to accurately assess p53 mutation status in tumor samples. Materials and Methods: A total of 102 tumor samples were collected from breast cancer patients referred to Tabriz hospitals between the 2007-2009 period. DNA was extracted by Proteinase K– Isopropanol method and then performed amplification and sequencing of P53 from exons 5 and 6. Results: Mutations in the P53 gene were detected in 17.6% of the patients. Including 7 polymorphisms (6.68%) and 11 mutations (10.78%). Overall, 18.2% of the mutations were found in codons 160 (ATG>AAG) and 163 (ATC>AAG) in exon 5. Also 81.8% of the mutations observed in exon 6: codon 193(CAT>AAT), codon195 (ATC>TTC), codon 195 (ATC>AAC), codon 198(GAA>TAA), codon 220 (TAT>TGT), codon 213 (CGA>CTA), and codon 214 (CAT>CG). No alteration observed in intron5 and all of polymorphism detected in 13399A>G nucleotide of exon 6. The majority of detected mutations are missense that located on DNA-binding domain of P53. This type of mutation usually leads to the production of a mutant protein with a compromised structure and altered DNA-binding capacity. Conclusion: This is the first report of its kind from the East Azarbaijan region. Our results indicate a rather high frequency of exon 6 mutations in P53 among patients with breast cancer. Furthermore, the mutation pattern appears differs from other regions. However, further studies are needed to determine the role of P53 mutations in breast cancer development.

R Eghdam Zamiri, M Moghimi, N Mosavi Nasab, H Amirmoghadami, M Joghatae , A Feizi,
Volume 20, Issue 81 (9-2012)

Background and Objective: Elevation of the b-HCG serum levels has been reported in several tumors including breast cancer, and it is usually associated with aggressiveness. The aim of this study was to examine the possible correlation between the b-HCG serum levels and different grades of breast cancer tumors in patients undergoing chemotherapy. Materials and Methods: This cross-sectional study was conducted in the city of Zanjan during 2009-10. Serum samples from 56 cases of breast cancer patients were collected after surgery and prior to chemotherapy for analysis of total free b-HCG by electro chemiluminescence immunoassay, and the same procedure was repeated after 8 courses of chemotherapy. The b-HCG serum levels were compared in poor versus mild to moderate grades before and after chemotherapy. Results: In 37 cases of mild to moderate grade tumors, the mean b-HCG level was 1.09 ±1.4 miu/ml compared with 1.2 ±0.3 miu/ml (P= 0.75) in 29 cases of poor grade tumors. The mean b-HCG levels before and after chemotherapy were 1.15 ±1.4 miu/ml and 1.17 ±1.4 miu/ml (P=0.24), respectively. Conclusion: We did not find any significant association between the b-HCG serum levels and breast cancer tumor grades. Furthermore, chemotherapy does not appear to have an effect on b-HCG serum levels.

V Ahmadzadeh, S Farajnia, Ma Hosseinpour Feizi, Ra Khavarinejad,
Volume 21, Issue 88 (7-2013)

Background and Objectives: Rituximab is an anti-CD20 chimeric monoclonal antibody widely used for the treatment of malignant B cells lymphoma. However, the immunogenicity of murine-derived monoclonal antibodies and the large size of full length antibodies restrict cancer immunotherapy. Humanized single chain antibodies can be a solution and a promising alternative for application in immunotherapy. The aim of this study was to produce a humanized scFv antibody for a potential use in the diagnosis and treatment of B cell lymphoma. Materials and Methods: We used a CDR grafting based approach to design a humanized scFv gene fragment. The CDRs were grafted onto the closest human frameworks. The designed sequence was expressed in E.coli then purified. The level of expression was analyzed by SDS-PAGE and the reactivity to CD20 expressing cell line was explored by immunoblotting. Results: Similarity analyses revealed that human germline gene IGHV1-46*03 and IGKV1-39*01 have the highest homology with their murine counterparts. Analysis by SDS-PAGE exhibited a high expression level in E. coli. Reactivity to CD20 expressing Raji cells showed that the produced antibody maintained the binding capacity to human CD20 marker. Conclusion: In our study, humanized anti- CD20 scFv indicated an original antigen-binding affinity. The findings serve as a basis for the development of novel therapeutic strategies in the treatment of CD20- expressing cancers.
F Sadeghi, M Khalaj-Kondori, Ma Hosseinpour Feizi, F Shaikhzadeh Hesari,
Volume 22, Issue 95 (8-2014)

Background and Objective: Learning is the acquisition of information about the world and memory is a mechanism to encode, store and retrieve the learned information. Weak memory and learning disorders are the most common cognitive problems. In the present study, the pharmacological effects of aqueous extract of Boswellia on learning and spatial memory in male rats was investigated using the Morris water maze (MWM) test. Materials and Methods: In this experimental study, 21 male rats were divided into 3 groups including a control group (distilled water) and two groups treated with aqueous extract of Boswellia (50 and 100 mg/kg) that received the treatment for 4 weeks. To evaluate learning ability of animals, Morris Water Maze was used. Results: In the first and the last day of training, all groups showed significant reduction in escape latency (P<0.0001) and traveled distance (P<0.0001). In the sixth day of training, both treatment groups showed significant reduction in escape latency (P<0.05) and traveled distance (P<0.05) in comparison with the control group. Conclusion: The results suggest that intake of Boswellia facilitates the learning and spatial memory formation in rats via Morris water maze test method. References 1- Sharifzadeh M, Sharifzadeh K, Naghdi N, Ghahremani MH, Roghani A. Posttraining intrahippocampal infusion of a protein kinase AII inhibitor impairs spatial memory retention in rats. J Neurosci Res. 2005 79(3): 392-400 2- Francis PT, Palmer AM, Snape M, Wilcock GK. The cholinergic hypothesis of Alzheimer’s disease: a review of progress. J Neurol Neurosurg Psychiatry. 1999 66(2): 137-47. 3- Abdel-Tawab M WO, Schubert-Zsilavecz M. Boswellia serrata: an overall assessment of in vitro, preclinical, pharmacokinetic and clinical data. Clin Pharmacokinet. 2011 50(6): 349-69. 4- Hussain H, Al-Harrasi A, Al-Rawahi A, Hussain J. Chemistry and biology of essential oils of genus boswellia. Evid Based Complement Alternat Med. 2013:140509. doi: 10.1155/ 2013/ 140509. 5- Moussaieff A, Mechoulam R. Boswellia resin: from religious ceremonies to medical uses a review of in‐vitro, in‐vivo and clinical trials. J Pharm Pharmacol. 2009 61(10): 1281-93. 6- Thulin M, Warfa A. The frankincense trees (Boswellia spp., Burseraceae) of northern Somalia and southern Arabia. Kew Bulletin. 1987: 487-500. 7- Sharma A, Mann A, Gajbhiye V, Kharya M. Phytochemical profile of Boswellia serrata: An overview. Pharmacog Rev. 2007 1(1): 137. 8- Poeckel D, Werz O. Boswellic acids: biological actions and molecular targets. Curr Med Chem. 2006 13(28): 3359-69. 9- Kimmatkar N, Thawani V, Hingorani L, Khiyani R. Efficacy and tolerability of Boswellia serrata extract in treatment of osteoarthritis of knee–A randomized double blind placebo controlled trial. Phytomedicine. 2003 10(1): 3-7. 10- Marshall S. Frankincense: festive pharmacognosy. Pharmaceutical J. 2003 271 (7280): 862-4. 11- Hosseini -Sharifabad M, Esfandiari E, Alaei H. Effects of frankincense aqueous extract during gestational period on increasing power of learning and memory in adult offsprings. Journal of Isfahan Medical School (iums). 2004 21(71): 16-20. 12- Hosseini-sharifabad M, Esfandiari E. Alaee, H. Moatar F. Effect of maternal consumption of aqueous extract of the gum resin of Boswellia serrata during lactation on increasing power of learning and memory in adult off springs. Iran J Basic Med Sci. 2003 6(3): 207-11. 13- Alaei H, Motahar F, Tory L. Effects of the abstract of oliban on learning and memory. J Ghazvin Univ Med Sci. 1999 21: 21-8. 14- Mahmoudi A, Hosseini-Sharifabad A, Monsef-Esfahani HR,et al. Evaluation of systemic administration of Boswellia papyrifera extracts on spatial memory retention in male rats. J Nat Med. 2011 65(3-4): 519-25. 15- Fathi FHH, Ali Hemmati AR, Banan Khojasteh SM. Effects of sesame oil on improving spatial memory in alzheimer's disease. J Babol Univ Med Sci. 2014 16(2): 34-41. 16- Hosseini M, Hadjzadeh MA, Derakhshan M, Havakhah S, Rassouli FB, Rakhshandeh H, Saffarzadeh F. The beneficial effects of olibanum on memory deficit induced by hypothyroidism in adult rats tested in Morris water maze. Arch Pharm Res. 2010 33(3): 463-8. 17- Hosseini M, Shafei MN, Safari V, Taiarani Z, Kafami Ladani M, Sadeghian R. The effects of olibanum administered to methimazole-treated dams during lactation on learning and memory of offspring rats. Nat Prod Res. 2012 26(16): 1544-8. 18- Hosseini-Sharifabad M, Esfandiari E. Effect of Boswellia serrata gum resin on the morphology of hippocampal CA1 pyramidal cells in aged rat. Anat Sci Int. 2014 Feb 11. [Epub ahead of print] 19- Jalili C, Salahshoor MR, Moradi S, Pourmotabbed A, Motaghi M. The therapeutic effect of the aqueous extract of boswellia serrata on the learning deficit in kindled rats. Int J Prev Med. 2014 5(5): 563-8. 20- Karima O, Riazi G, Yousefi R, Movahedi AAM. The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study). Neurol Sci. 2010 31(3): 315-20. 21- Lee S-C, Moon Y-S, You K-H. Effects of red ginseng saponins and nootropic drugs on impaired acquisition of ethanol-treated rats in passive avoidance performance. J Ethnopharmacol. 2000 69(1): 1-8. 22- Savelev S, Okello E, Perry N, Wilkins R, Perry E. Synergistic and antagonistic interactions of anticholinesterase terpenoids in Salvia lavandulaefolia essential oil. Pharmacol Biochem Behav. 2003 75(3): 661-8. 23- Singh G, Atal C. Pharmacology of an extract of salai guggal ex-Boswellia serrata, a new non-steroidal anti-inflammatory agent. Agents Actions. 1986 18(3-4): 407-12. 24- Dhingra D, Parle M, Kulkarni S. Memory enhancing activity of Glycyrrhiza glabra in mice. J Ethnopharmacol. 2004 91(2): 361-5. 25- Mayford M, Kandel ER. Genetic approaches to memory storage. Trends Genet. 1999 15(11): 463-70. 26- Tully T, Bourtchouladze R, Scott R, Tallman J. Targeting the CREB pathway for memory enhancers. Nat Rev Drug Discov. 2003 2(4): 267-77.

Mr Aliparasti , M Dadkhah , M Alipour , Sh Almasi, H Feizi ,
Volume 24, Issue 106 (7-2016)

Background and Objective: Ghrelin has different functions in the body and one of its newly known roles is the antiapoptotic effect. However, this effect of ghrelin has not been considered in the probable hypoxia induced apoptosis in the animal lung tissue. The aim of this study was to examine the effect of ghrelin treatment on Bax/Bcl-2 gene expression in the lung tissue of rats with chronic hypoxia.  

Materials and Methods: Twenty four male Wistar rats were divided in three experimental groups of normal, hypoxic+saline and hypoxic+ghrelin and were treated for 2 weeks. The expression level of Bax and Bcl-2 genes were assessed using Real-Time PCR in isolated lung tissues of all animals. In addition, histological changes of pulmonary arteries were examined following Hematoxylin-Eosin staining of the isolated tissues.

Results: Chronic hypoxia caused pulmonary artery wall thickness and treatment with ghrelin reversed the changes to the normal. Keeping animals under chronic hypoxia treated or not with ghrelin had no significant effect on Bax/Bcl-2 gene expression ratio as measured in the total lung.

Conclusion: Ghrelin treatment restores the histological changes induced by chronic hypoxia in rats. However, no significant changes in Bax/Bcl-2 gene expression ratio as a marker of cell apoptosis seem to occur in the isolated lung tissues, even after ghrelin treatment. Further similar studies especially in vascular endothelial and smooth muscle cells are recommended.

Ma Hosseinpour Feizi, N Dastmalchi, N Pouladi, R Safaralizadeh, P Azarfam,
Volume 25, Issue 112 (7-2017)

Background and Objective: Breast cancer is the most common cancer and the major cause of cancer death among women. The survival rate for breast cancer indicates the percentage of people that survive the disease for a specified period of time after diagnosis. The aim of this study was analyzing the survival rate of patients with breast cancer in East Azarbaijan province.

Materials and Methods: This study was a retrospective analysis. The statistical population included 79 breast cancer patients of East Azarbaijan province. Data such as age at diagnosis was obtained from the medical records and other data such as the status of survival/death was found out through telephone interview. For survival evaluation, the Kaplan-Meier method was used. The effect of covariates such as age at diagnosis on the survival duration was calculated with the Log - Rank (Mantel - Cox) method.

Results: The 1, 3 and 5 year-survival rate were calculated to be 98%, 88% and 82%, respectively. Age at the time of diagnosis affected the survival rate of patients; patients over 40 years at the time of diagnosis had a higher survival rate than patients under 40 years (p<0.05).

Conclusion: Survival rate analysis showed the similar or higher survival rate of breast cancer patients of East Azarbaijan in comparison with other regions in Iran. The survival rate in patients older than forty years at the time of diagnosis was significantly higher than patients younger than forty. However a study on more patients is recommended to obtain more clear results.

Ar Abdanipour, B Shahsavandi, M Dadkhah, M Alipour, H Feizi,
Volume 25, Issue 113 (8-2017)

Background and Objective: Apoptosis is a form of programmed cell death and oxidative stress is one of the factors that lead to apoptosis. Ghrelin is a 28 amino acid peptide and its antiapoptotic effects have been shown previously. The aim of this study was to evaluate the effect of ghrelin on cell survival and apoptosis of rat bone marrow mesenchymal stem cells (BMSCs).
Materials and Methods: BMSCs were cultured as control and H2O2 treated groups with and without different doses of ghrelin (0.1-1-10-100 µM) for 24 and 48 hours. To find out the best dose and time of effect for ghrelin, MTT was used to determine cell survival. BMSC apoptosis rate was detected using the TUNEL technique.
Results: Cultured BMSCs showed that the cells are fibroblastic in appearance and adherent to culture dishes as well as being capable of having a very high proliferation rate. Ghrelin treatment increased proliferation and cell survival in both normal and H2O2 exposed BMSCs. The best dose and time that ghrelin could affect cell proliferation was 100µM and 48 hours respectively. Treatment with ghrelin significantly reduced apoptosis in BMSCs in this dose and time.
Conclusion: Ghrelin treatment causes an increase in BMSCs proliferation and reduction of H2O2 induced apoptosis in these cells.

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