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Showing 12 results for Zare

B Amini, M Kamali, A Zarei Mahmod Abadi, Y Mortazavi, A Ebrahim Habibi, E Bayat, N Farhadi, Hr Javadi, Ah Kyhan,
Volume 18, Issue 71 (5-2010)

Background and Objective: Antibody against Pseudomonas aeruginosa exotoxin A can be used in immunotherapy together with antibiotics to treat acute burn patients. Exotoxin A is one of the virulence factors in Pseudomonas aeruginosa that comprises of three domains, binding domain, translocation and catalytic domain. The purpose of this study was to produce recombinant domain of the catalytic part of this microorganism in order to produce antibody against it. Methods and Materials: Pseudomonas aeruginosa samples were isolated from burn patients hospitalized in Mousavi Hospital, Zanjan, Iran and Pseudomonas aeruginosa species were identified by Biochemical tests. Bacteria genomic DNA was extracted and exotoxin A gene determined by PCR. Catalytic domain of exotoxin A was amplified by PCR. Products and plasmid extracts was digested by restriction enzymes. Subsequently PCR products and plasmids transformed into E. coli BL21 (DE3). Clones containing gene of interest was determined by PCR, restriction enzyme and sequencing. Results: The sequence homology of the catalytic domain of exotoxin A was compared with that of the published gene data bank. The results showed a complete homology between our gene species and the published genome in data banks. Conclusion: The results of this study showed that about 90% of the isolated bacteria contained exotoxin A and there was a sequence homology between our species and published gene data banks.

Ms Aghvamy, Sh Mohammadzadeh, M Gallalmanesh, R Zare,
Volume 19, Issue 74 (3-2011)

Background and Objective: Asthma is one of the chronic diseases of childhood that affects patients' quality of life. Planned education is considered as an important factor in management of sufferers from chronic diseases. Subsequent implementation of such programs leads to almost 70% decrease in chronic diseases and reduction of the confined- to- bed individuals. The aim of this study was to explore the effect of group vs. computer education on the quality of life in 8-12 year old children suffering from asthma in Zanjan Vali-e Asr Hospital. Materials and Methods: This quasi- experimental study was conducted in 80 children aged 8-12 years who were suffering from moderate to mild asthma. Data collection tool was a questionnaire in which 11 items were about individual characteristics and 25 items were about quality of life concerning physical, psychological, mental and social dimensions. Samples were selected by a non- random method and then they were randomly divided into two categories group and computer education. The questionnaires were completed by the researcher before and after the intervention. After one month the data were collected and then analyzed statistically. Results: According to the analyses, mean and ranking of the quality of life in our study, regarding WHO standards, indicated an increase in the quality of life and there was a significant increase after the intervention in the quality of life in both groups (p<0/05). Conclusion: The findings showed that not only education in both ways was helpful in the improvement of the quality of life, but the computer based education had an outstanding impact on it. Therefore, perseverance and promotion of the quality of life by means of continuous computer education for these patients are recommended.

N Hamid, R MaZarei, S Pak,
Volume 21, Issue 84 (3-2013)

Background and Objective: The aim of this research was to study the correlation between daily stresses and coping styles with mental health in migraine headache patients. Materials and Methods: The research method was comparative-casual method. The sample consisted of 60 patients with migraine headaches (30 males and 30 females) as the test group, whom were matched for various criteria including age, educational status, and suffering from headache for at least a two year period. The control group consisted of 60 healthy school teachers (30 males and 30 female). The instruments included Lazarous–Folkman Coping Style, Holms-Rahe life stress, and General Health questionnaires. The data were analyzed with the logistic regression method. Results: The results revealed that there was a significant difference between the test and control groups in daily stresses and coping styles (p<0.001). Furthermore, the rate of stresses in patients with migraine headache was significantly higher than the normal subjects. In addition, the rate of emotional styles in patients with migraine headache was higher than normal controls. Conclusion: There was a negative correlation between daily stresses, emotional styles, and mental health in patients with migraine headache.

M Soofy Majied, F Gharibi, M Zarei,
Volume 21, Issue 85 (4-2013)

Background and Objective: low-value junk food consumption due to the large amount of salt, fat and sugar, prepare chronic diseases (heart diseases - cardiovascular, obesity, and hypertension) in adult age. This study has been designed to investigate the effect of health education methods on students' knowledge and practice of nutrition in junk food consumption. Materials and Methods: This quasi-experimental study was carried out on 618 (the fifth grade) elementary school students in regions 1 and 2 schools of Sanandaj in 1391-1390 academic year. 246 students were in the film show group, 204 students in lecture group and 168 students in control group. In film group, a film was showed 45 minutes for students. In lecture group, in order to follow similarity a text of the same content which showed in the film prepared and presented for 45-minutes by health expert. Students in the control group did not receive any training. Knowledge and practice of students was measured by questionnaires which included demographic questions, Knowledge questions and practice questions. Results: The sample age was 10 to 12 years with average 11.07 ± .58.46 percent of the population were female and 54 percent were male. The results showed that Knowledge and practice on junk food consumption in three groups after training had a significant difference (P = 0.0001), also in lecture and show film groups before and after intervention there was a significant difference (p = 0.0001). The Knowledge and practice of the control group was not significant before and after training. Conclusion: The results showed the positive effect of indirect educational methods (showing film) on practice on junk food consumption and also revealed that the impact of showing film is greater than the lecture.

M Zare, A Zar, Ma Edalatmanesh,
Volume 24, Issue 103 (4-2016)

Background and Objective: Alzheimer is a neurodegenerative disease in which a reduced memory capacity and numerous changes occur in the patient’s brain. This study was embarked to shed light on the effects of eight weeks of endurance training and lithium chloride administration on brain-derived neurotrophic factor serum levels in female rats with Alzheimer's disease.

Materials and Methods: 70 female Sperag Dawley rats were randomly divided into seven groups including: 1.control, 2. Sham, 3. littium chlorid[d1] e 20, 4. littium chloride 40, 5. Endurance training, 6. Endurance training littium chloride 20, 7. Endurance training and littium chloride 40. Alziemer was induced in rats by methyl chloride,. Groups of endurance training, endurance training plus littium coloride 20 and endurance training plus littium chloride 40 ran on a treadmill with no incline at the speed of 15 to 20 meters per minute for 15 to 30 minutes each session and five times for eight weeks. The groups of littium coloride 20, endurance training and littium coloride 20 received littium coloride 20 mg/kg according to their body weight per day. The littium coloride 40, endurance training and littium coloride 40 received  littium coloride 40 mg/kg of their body weight per day for 14 days.  During this period, the control group did not have any activity and did not recieve any treatment. ANOVA and tukey post hoc[d2]  tests were used for statistical analysis.

Results: The results showed that lithium chloride as well as endurance training along with their  interactive effects increased levels of brain-derived neurotrophic factor poisoning by neurotoxins. But the greatest impact was observed in endurance training and lithium chloride 20. Also, serum levels of brain-derived neurotrophic factor significantly decresed owing to Methyltin[d3]  poisoning in female rats (p<0.05).

Conclusion: Based on these findings, we conclude that lithium chloride, endurance training and their interactive effects in female rats with Alzheimer's disease raised brain-derived neurotrophic factor serum levels which implies that Alzheimer's disease can be improved.

A Jokar, F Zynali, M Akbarzade, N Zare,
Volume 25, Issue 108 (3-2017)

Background and Objective: The importance of menopause is due to the onset of menopausal symptoms and complications. Hot Flushes are one of the most common and earliest of them, for which treatments such as hormone therapy and non-pharmaceutical methods have been proposed. In this study due to the tendency of most patients in choosing non-pharmaceutical methods, we evaluated the effect of acupressure on hot flushes.

Materials and Methods: This clinical trial was randomly conducted on 90 subjects (30 in KI-1, 30 in LI-4, and 30 in HT-7 groups) selected through purposive sampling in Shahid Motahari clinic, Shiraz in 2014. Acupressure was applied on KI-1, LI-4, and HT-7 acupoints by rotational method twice a week each time for 5 minutes for 5 weeks. The position of the KI-1 acupoint is located on the sole of the foot, at the junction of the anterior 1/3 and posterior 2/3 of the line connecting the bases of the second and third toes with the heel. The HT-7 acupoint is located at the wrist crease (on the radial side of the flexor carpi ulnaris tendon) between the ulna and the pisiform bones. Afterwards, the data were entered into the SPSS statistical software (v.16) and analyzed using ANOVA.

Results: The results showed a significant difference in the intensity of hot flashes in LI-4 and KI-1 groups (P<0.001), but no significant change was observed in the control group (P=0.233). Moreover, no significant difference was observed within the three groups regarding the duration of hot flashes (P>0.05). Nonetheless, a significant difference was found between the intervention groups and the control group with respect to the frequency of hot flashes (P<0.001).

Conclusion: Our results show that acupressure is significantly effective in treating hot flashes. Therefore, acupressure can be recommended as an inexpensive and easily applicable treatment method.

P Zareie, M Sadegh, Mr Palizvan,
Volume 25, Issue 109 (4-2017)

Background and Objective: In spite of various and effective anti seizure drugs available, 30% of epileptic patients are not adequately treated with current medications. Based on the effectiveness of phytocannabinoids on epileptic and seizure models, in this study the effects of 2-achidonoylglycerol (2-AG) injection, as an important endocannabinoid, on tonic-clonic seizures induced by pentylenetetrazol (PTZ) was examined.  

Materials and Methods: The study was performed on male wistar rats (180-200 g). Tonic-clonic seizures were induced through a single intra-peritoneal injection of PTZ (80 mg/Kg) and then seizure behavior was monitored for 30 minutes. The intra-peritoneal injection of 2-AG (1 mg/Kg) in dimethyl solfoxide (DMSO) was performed 15 minutes before the PTZ injection. In the sham group an equivalent volume of DMSO was injected 15 minutes before PTZ. Data on the delay of seizure stage occurrence, duration of the stages, number of occurrences for each stage and mortality rate due to tonic-clonic seizures were collected for analysis.

Results: PTZ injection in association with DMSO significantly increased the delay for seizure stages 1 and 2, in comparison with just a PTZ injection (P<0.05), but had no significant effect on the duration and delay of seizure stages 3-5. Intra-peritoneal injection of 2-AG in association with DMSO before the injection of PTZ had no significant effect on the delay and duration of seizures but reduced the occurrence of each seizure stage and also reduced mortality due to tonic-clonic seizures in comparison with PTZ injection associated with DMSO. The mean seizure stage was also significantly decreased (P<0.05).

Conclusion: It seems 2-AG injection could be effective in reducing seizure parameters in seizures induced by PTZ.

M Efati, M Khorrami, A Zarei Mahmoud Abadi, J Raouf Sarshoori,
Volume 25, Issue 110 (5-2017)

Background and Objective: Fatty liver disease occurs due to disturbances in lipid metabolism (increased fat synthesis and halting of catabolism) which eventually leads to the accumulation of large amounts of fat (triglycerides) in liver cells, that if left untreated lead to inflammation and cirrhosis. This study aimed to investigate the effect of concomitant use of HESA-A and atorvastatin on biochemical parameters in non-alcoholic fatty liver disease compared with atorvastatin alone.

Methods and Materials: 28 rats weighing 180±20 g were randomly divided into two groups: a control group (n = 7) with standard diet and a HFD (high-fat diet) group (n=21) which were fed for 8 weeks. To confirm the induction of fatty liver; controls along with seven rats from the HFD group were killed. The remaining 14 animals were divided into two groups. The first group received atorvastatin and the second group received HESA-A in combination with atorvastatin daily for 30 days. Finally, biochemical parameters (glucose, lipid profile and liver enzymes) were measured and histological studies were performed on the liver samples.

Results: Biochemical parameters in the HESA-A combined with atorvastatin group were significantly decreased in comparison to the atorvastatin group (p<0.01). The histopathological study of liver tissue showed a significant reduction in the amount of fat tissue and conversion of fatty liver from grade ІІІ to grade І.

Conclusion: According to the results of this study, the use of HESA-A as a complementary treatment for fatty liver disease without side effects can be integral role in reducing the incidence of non-alcoholic fatty liver disease, especially in severe cases.

S Azima, S Houshyar, M Motamedi Far, M Kaviani, N Zare,
Volume 26, Issue 114 (3-2018)

Background and Objective: Generally, 75% of women experience vaginal candidiasis at least once in their lifetime. This study aimed to determine the effect of lactobacillus rhamnosus vaginal probiotic capsule on vaginal colonization and treatment results after treating vulvar and vaginal candidiasis.
Materials and Methods: This double-blind clinical trial was conducted in 58 women suffering from vulvar and vaginal candidiasis (based on swab cultures). After treating the patients with clotrimazole 2% vaginal cream, the samples were randomly assigned to probiotic (n=28) and placebo (n=30) groups. While first group took lactobacillus rhamnosus vaginal probiotic capsules twice a day for one week, the second group used vaginal placebo capsules. The patients were examined regarding swab cultures, vaginal lactobacillus colonization, vaginal pH, and clinical symptoms of vulvar and vaginal candidiasis 2-5 days before and 1 month after taking the capsules. Then, the data were fed to SPSS and analyzed using Chi-square, Fisher’s exact test, repeated measures ANOVA, and post-hoc test.
Results: The results showed no significant difference between the two groups concerning the results of fungal culture. Moreover, despite the increase in vaginal lactobacillus colonization in the probiotic group, no significant difference was found between the two groups at different time periods (p=0.255). The results also showed no significant difference between the two groups regarding the mean change of vaginal pH at different time periods (p=0.480).
Conclusion: It seems that using vaginal lactobacillus rhamnosus probiotic capsules was somewhat effective in vaginal lactobacilli colonization in patients with vaginal candidiasis. However, it was not effective in pH changes and treatment results.
Ms Parisa Zareie, Dr Mehdi Sadegh, Dr Homeira Moradi-Chameh,
Volume 26, Issue 118 (September & October 2018)

Background and Objective: 2-arachidonoylglycerol (2-AG) and anandamide (AEA) are two major endocannabinoids. Using inhibitors of the enzymatic pathways involved in the elimination of 2-AG and AEA as well as synthetic 2-AG, we examined the effectiveness of these endocannabinoids on epileptiform activity induced in Wistar rats by pentylenetetrazol (PTZ).
Material and Methods: Adult male Wistar rats were used in this study. Epileptiform activity was induced in dult male Wistar rats by PTZ injection (20 mg/kg, i.p.). To inhibit 2-AG degradation WWL70 and JJKK048 (JJKK048: 1 mg/kg, WWL70: 5 mg/kg, i.p.) were used. To inhibit AEA elimination, URB597 and LY2183240 (URB597: 1 mg/kg, LY2183240: 2.5 mg/kg, i.p.) were used. Synthetic 2-AG was also examined (1 mg/kg, i.p.) before the PTZ injection. All drugs were dissolved in DMSO as vehicle and injected (i.p.) 15 minutes before the PTZ injection. Latency to onset and duration of the epileptiform activity were considered for statistical analysis.
Results: Injection of (JJKK048+WWL70) before the PTZ significantly increased latency to onset of the epileptiform activity (p<0.01), while reduced duration of the epileptiform activity in comparison to the vehicle (p<0.05). In addition, 2-AG administration significantly increased latency to onset of the epileptiform activity (p<0.05) and reduced duration of the epileptiform activity in comparison to the vehicle (p<0.01). However, these indexes did not show significant changes when URB597+LY2183240 were injected before the PTZ (p>0.05).
Conclusion: It seems increased level of 2-AG but not AEA,effectively decreases PTZ induced epileptiform activity of the hippocampus.

Parvane Mohammadi, Mehdi Alizadeh Zarei, Samanehh Karamali Esmaili,
Volume 26, Issue 118 (September & October 2018)

Background and Objective: Children with Autism spectrum Disorder (ASD) are limited in participation in everyday activities due to the nature of their disorder. Participation means involvement in life situations. The aim of this study was to evaluate psychometric properties of “the Children Participation Assessment Scale-Parent Version” (CPAS-P) in activities outside of school in Iranian children with ASD.
Material and Methods: The sample consisted of 55 parents of 6-12 years old children with ASD who selected with convenience sampling. For assessing the convergent validity of CPAS-P and Vinland Adaptive Behavior Scale (VABS), the spearman's correlation coefficient test was used. The internal consistency and test-retest reliability were determined by using the Cronbach's alpha and the intraclass correlation coefficient (ICC), respectively.
Results: The mean age of children was 7.62 years. Cronbach's alpha was obtained 0.91 to 0.94, which indicates the proper internal consistency and ICC was 0.90 to 0.95, which indicates the acceptable test-retest reliability. Finally, significant correlations were reported between CPAS-P and VABS tests in related subscales.
Conclusion: Increasing independence in doing daily activities of children with ASD is a concern for rehabilitation professions such as occupational therapists. Valid and reliable instruments are necessary for documenting treatment that should culturally adapted among Iranian children. According to the result of this study, CPAS-P has good psychometric properties for clinical practice and research.

Milad Rajabi, Parvin Zareian, Mino Shaddel, Iraj Karimi,
Volume 27, Issue 125 (November & December 2019)

Background and Objective: Skin ulcers and their slow healing are considered as one of the important medical aspects. Nowadays, various antibiotics and materials are used to accelerate the wound healing process. One of the methods taken in the field of wound healing is using ozone. On the other hand, one of the factors that hurt the speed of wound healing is stress. Therefore, the present study aimed to assess the effect of ozonated water on healing of skin ulcers in stressful conditions.
Materials & Methods: A total of 80 male mice were randomly divided into four groups: stress -no ozone, stress - ozone, no stress-no ozone, no stress- ozone group. Isolation stress was used (daily, 4 hours, for 14 days). The healing process was evaluated based on measuring the wound surface area in days 2, 6, 10, and 14 and histopathological data.
Results: Isolation stress by decreasing the fibroblast maturation and angiogenesis in the wound leads to a reduction in the speed of wound healing. Ozone enhanced the rate of tissue repair in both stress and stressless situations by decreasing inflammation and increasing the fibroblast maturation, amount of collagen tissue, rate of re-epithelialization, new vascularization, and granulation tissue size.

Conclusion: The results of this study implied that treatment with ozone could accelerate the wound healing process and decrease the harmful effects of stress conditions in healing process of animal model.

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