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Showing 9 results for هانیلو

Volume 3, Issue 9 (12-1994)

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Volume 8, Issue 32 (9-2000)

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Volume 12, Issue 46 (Mar 2004)

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Volume 12, Issue 49 (Dec 2004)

Background: Mosquitoes are the most important blood feeding insects that can transmit serious diseases such as malaria and arboviruses. Each species of mosquitoes selects a specific host according to biological conditions of the region and genetic characteristics. Anopheles maculipennis complex and Culex theileri are dominant mosquito species in Zanjan. This study was carried out in order to determine the blood feeding index of mosquitoes by Sanduwich Enzyme Linked Immunosorbent Assay (ELISA) in 2004. Materials and Methods: An. maculipennis complex and Cx. theileri mosquitoes were collected from indoors and outdoors by pyrethrum spray catch (PSC) and light trap in 2003 and blood fed samples were separated and kept at -20°c subsequent to drying. The samples were tested for human blood after establishing Sandwich ELISA technique. The results were analyzed through test. Results: A total of 6436 samples were studied, out of which 3072 (47.73%) were An. maculipennis and 3364 (52.3%) Cx. theileri. Human blood-feeding index in An. maculipennis complex was 3.2% while this rate reached 1.5% in Cx. theileri. An. maculipennis complex showed 2.12% more anthropophilic behavior than Cx. theileri. Anthropophilic index of this anopheles by PSC was 0.2%. This index was 7.3% (93) in samples collected by light trap. There was a significant difference in two rates (p=0001). In PSC samples anthropophilic index was 35.17 times less than light trap samples. Anthropophilic index of this Culex was 1.8% through PSC and 1.3% through light trap, which shows no significant difference. Conclusion: With regard to high human blood feeding index of An. maculipennis complex, we suggest that health officials of the province pay careful attention to malaria control and monitoring programs. To reduce human blood feeding index people should be encouraged to use personal protection and keep livestock to divert mosquitoes. More research associated with molecular biology, ecology and bionomy is recommended to determine this difference.

P Rahimi, Mb Ghavami, A Haniloo, A Nourian, Ar Biglari,
Volume 16, Issue 65 (12-2008)

Background and Objective: Fascioliasis is an important zoonotic disease that causes several health problems and economical losses in different parts of Iran including Zanjan. Fasciola hepatica and F. gigantica are recognized as causative agents of the disease. The differential diagnosis between these two species is very important for planning and control of infection. This study was designed to identify the Fasciola species by molecular methods in Zanjan (Iran). Methods and Materials: A number of 535 adult Fasciola worms were collected from the natural infected livers of cattles and sheep in local slaughterhouse. Living flukes were washed extensively in PBS at 37 OC and then anterior half of adult worms were stored at -20 OC in 70% ethanol. Total genomic DNA was extracted from individual flukes by modified phenol-chloroform method. Nucleotide polymorphism of ITS2 fragment of rDNA was investigated using PCR-RFLP assay and sequencing technique. Results: The results of PCR-RFLP and comparison of ITS2 sequences with the BLAST GenBank database clarified that all specimens were F. hepatica. The obtained sequences are available in the GenBank, with accession numbers EU391412 to EU391424. Conclusion: The results of this study showed no evidence of F. gigantica infection in sheep and cattles in Zanjan as all of the isolates were found to be F. hepatica. Key words: Fasciola, Liver flukes, rDNA, ITS2, PCR-RFLP

A Haniloo, F Najafi, A Fazaeli, Aa Nourian,
Volume 19, Issue 74 (3-2011)

Background and Objective: Preparation of proper antigens is an important issue in serology of hydatidosis. Investigators have been able to obtain excretory/secretory antigens (E/S Ags) by short-term culture of protoscoleces in a couple of culture media. However, no data are available about production rate of such antigens in different culture media. The present study was carried out to evaluate the production of E/S Ags (proteins) in PBS complemented with glucose, DMEM and RPMI culture media. Material and Methods: To obtain E/S proteins, protoscoleces of echinococcus were cultured in PBS complemented with 10% glucose, RPMI and DMEM for 72 hours. Proteins secreted in culture media were concentrated and assayed. To characterize different components, proteins were electrophoresed on SDS-PAGE. Data were analyzed using One-way ANOVA and Tukey HSD tests. Results: The mean concentration of E/S proteins in PBS medium in 24 hours of culture was significantly higher than DMEM and RPMI (P<0.05). However, such a difference was not observed between E/S proteins in DMEM and RPMI media. E/S proteins obtained from PBS medium were separated into 12 major bands and the two other media into 14 major bands within a range of molecular masses of 16 to 67 kDa. Conclusion: PBS complemented with glucose is more appropriate than the two other media for E/S proteins production. The best time to obtain E/S proteins is the first 48 hours of culture.

A Haniloo, M Farhadi, A Fazaeli, N Nourian,
Volume 21, Issue 84 (3-2013)

Background and Objective: Hydatidosis is one of the most important zoonoses caused by larval stage of Echinococcus granulosous which is distributed worldwide. Determination of the parasite genotypes in relation to the host specificity and transmission routes is of great importance. So far, DNA based analysis of the isolates obtained from different host species has lead to identification of 10 different genotypes (G1-G10) in the world endemic areas. This work was designed to identify the genotypes of the hydatid cysts isolated from domestic animals and people in Zanjan. Materials and Methods: A total of 86 isolates, including 49 sheep, 28 cattle isolates collected from the slaughter houses, and 9 human isolates collected from hospitals were subjected to genotype analysis using PCR-RFLP of the ribosomal DNA ITS1 region. Results: A fragment of about 1000 bp was amplified from all samples using ITS1 PCR. The endonuclease digestion of the PCR products resulted in the RFLP pattern that were the same for all isolates as the pattern expected for the sheep strain. The restriction patterns of all 4 enzymes used ( Alu I, TaqI, RsaI, and Msp I), consistently approved the same genotype. Conclusion: The PCR RFLP pattern obtained from our samples were characterizes as E. granulosous sensu stricto, which is the same as what has been previously reported in Iran. It can be concluded that the hydatid cyst isolates in our study area, Zanjan, is basically similar to that of other endemic areas of Iran.

R Norouzi , A Nourian, A Hanilo , K Kamali,
Volume 24, Issue 102 (3-2016)

Background and Objective: High prevalence of parasitic infections can be the result of asymptomatic infections. A number of regular and ongoing epidemiological studies are prerequisites in order to control these infections. This study was carried out with elementary school students to achieve an overview of the spread of parasitic infections in Zanjan city, Iran.

Materials and Methods: In this cross-sectional study, using random cluster sampling, 854 of 6-12 year-old students were selected from 10 primary schools in Zanjan by three methods of direct smear, formalin-ether concentration and Scotch tape.

Results: Overall, 139 students (16.3%) were found to be infected by intestinal parasites. The protozoa Entamoeba coli (4.56%), Chilimastix mesnili (4.33%), Blastocystis hominis (3.04%), Giardia lamblia (2.34%), Iodamoeba butschlii (1.87%), Enterobius vermicularis (1.52%), Endolimax nana (0.46%), Dientamoeba fragilis (0.23%), Hymenolepis nana (0.11%), Sarcocystis (0.11%) were the most common parasite types. There was a significant difference in parasite prevalence between age and parents’ education. However, no significant difference was found with gender, family income, and residence.

Conclusion: The results indicated that the prevalence of intestinal parasitic infections in primary schools was in low level and in comparison with 1994’s study in Zanjan city it has significantly decreased. With regard to the prevalence of intestinal parasites in study areas, improvement of health awareness among students and parents especially in the field of personal health seems necessary.


S Ahang , A Haniloo , S Faghih Zadeh,
Volume 24, Issue 103 (4-2016)

Background and Objective: Current and common treatment of toxoplasmosis consist of oral adminstration of pyrimethamine and sulfadiazine. However, this drug combination has substantial toxicity and side effects on patients, especially in immunocompromised individuals. Echinacea purpurea is a medicinal plant with antimicrobial properties which boosts the immune system. This study was carried out to evaluate the anti-toxoplasma effect of E. purpurea extract in vitro and acute toxoplasmosis in a mouse model.

Materials and Methods: Parasite suspension containing 42000 live toxoplasma tachyzoites (RH strain) was added to serial concentrations of 50, 100, 200, 500 and 1000 µg/ml alcoholic extrac of E. purpurea and incubated at 37oC for 5 hours. Then, IC50 was calculated using linear regression. Subsequently, 48 BALB/c mice were asssigned to 5 treatment groups and a control group and were intraperitoneally infected with 104 tachyzoites. 24 hours post infection, the E. purpurea extract was injected intraperitoneally by the aforementioned concentrations on a daily basis until the death of the mice. The control group received sterile saline. Survival time of the groups was analyzed by One way ANOVA and Duncan tests and between-group differences at the level of P<0.05 were considered statistically significant.

Results: Using linear regression, IC50 values of E. purpurea extract was 784 µg/ml, after 5 hours. The mean survival time of the mice in the treated groups was significantly higher than the control group, respectively 6.5±0.9 and 5±0.0 days (P<0.05). The maximum survival time (7.3±1.0) without any significant difference with other test groups, was obtained in the fifth group (1000 &mug/ml).

Conclusion: The tested concentrations of alcoholic extract of E. purpurea did not pose any inhibitory effect on tachyzoites in vitro, but significantly increased the survival time of  the infected mice in vivo.

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