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Showing 3 results for کاکا

Z Abdi, Sh Sadraie, Gh Kaka, M Jafari, M Saberi, Mh Hossini Akbari, H Dashtnavard, A Pandoeh,
Volume 16, Issue 65 (12-2008)

Background and Objective: Sulfur mustard is a potent chemical vesicant warfare agent that remains a significant military and civilian threat. Inhalation of sulfur mustard gas causes inflammation and injury to airways and bronchioles. Mast cells promote allergic reactions when exposed to some chemical compounds such as HD. Hexamethylenetetramine has been shown to protect human lung cells against HD toxicity and has also been shown to be effective against the chemical warfare agent phosgene in vivo. The aim of this study was to evaluate the effects of HMT on the number of mast cells in the lamina propria of visceral layer of pleura in male rats after exposure to sulfur mustard. Materials and Methods: Twenty seven Albino Wister male rats weighting 200±20 gr were randomly divided into 5 groups (Normal Saline (N.S), HMT, HD, Pre-exposure and Post-exposure). HD, Pre-exposure and Post-exposure groups received sulfur-mustard and N.S group received Normal Saline as a solvent by intratracheal catheter. HMT, Pre-exp and Post-exp groups received HMT via intra-peritoneal for 14 days. After the day 14, body weight changes, the rate of lung tissue injury and the number of mast cells measured in the pleura's visceral layer of the rats' lungs. Results: Histological examination and mast cells count showed no significant difference when compared among NS, HMT, Pre-exposure and Post-exposure groups. However, significant reduction was seen in the number of mast cells in HMT and NS groups in comparison with the HD group (p < 0/001). The number of mast cells in the Pre-exposure and Post-exposure groups was also significantly lower than that of the HD group (p < 0/001). Conclusions: From the results of this study it can be concluded that HMT may have a positive protective and therapeutic effect on lung tissue in rats. Key words: Sulfur mustard, Lung, Mast cell, Hexamethylenetetramine, Rat

D Zolfagari, G Kaka, M Sadri, Sh Sadraie, A Emamgoli, M Asghari Jafarabadi, Gh Herfedoost,
Volume 22, Issue 92 (5-2014)

Background and Objective: Poly D,L-lactic-co-glycolic acid (PLGA) is known as biodegradable and biocompatible polymer. These polymers have recently received much attention in tissue engineering. The aim of this study was to investigate the biological behavior of the bone marrow stromal cells (BMSCs) in culturing with PLGA nanofibers coated with poly-L-lysine or gelatin. Materials and Methods: In this study, the electrospinning of PLGA nanofibers was performed with hexafluoro-2-propanol (HFIP) as solvent and coated with gelatin and poly-L-lysine, separately. The properties of polymers were investigated with scanning electron microscopy (SEM) analysis and contact angles were studied. After culturing BMSCs and reaching passage two, cells in the four groups of nanofibers, nanofibers PLGA, PLGA nanofibers coated with poly-L-lysine, and PLGA nanofibers coated with gelatin, were grown. Cell proliferation during the second, fourth and sixth days were examined by Acridin Orange and morphology of the cells seeded on nanofibers were investigated via SEM. Results: The mean diameter of PLGA nanofibers were between 270 - 700 nm. The average contact angles were 107.66° for PLGA, 64.58 for PLGA coated with gelatin and 40.12 for poly-L-lysine-coated PLGA, respectively. The results showed significant reduction in cell proliferation in PLGA nanofibers alone (P <0.05). But this number increased in groups of nanofibers coated with poly-L-lysine and gelatin. Conclusion: Culture of Schwann cells with PLGA nanofibers coated with gelatin and particularly coated with poly-L-lysine provide a biodegradable scaffold associated with Schwann cells.

G Yazdanpanah, M Kakavand, H Niknejad,
Volume 23, Issue 99 (6-2015)

Background and Objective: Amniotic membrane (AM) as a natural tissue has lots of unique features which make it a suitable candidate for vascular tissue engineering. The aim of this study was to evaluate blood compatibility of mesenchymal surface of the AM. Materials and Methods: In this study, the effect of mesenchymal surface of the AM on internal and external coagulation pathways, hemolysis and platelet activity was measured and the results were compared with heparin-coated ePTFE as a synthetic vessel substitute. In addition, platelet adhesion and their morphologic changes after being in contact with samples were analyzed by electron microscopy. Results: Prothrombin time (PT), activated partial thromboplastin time (aPTT), clotting time and hemolysis tests showed that the AM is hemocompatible. Additionally, mesenchymal surface of the AM induced platelet aggregation less than ePTFE while both similarly provoked secretion of P-selectin. Number of adhered platelets to mesenchymal surface of the AM was less than ePTFE. The platelets on the mesenchymal surface had round morphology whereas ePTFE exhibited dendritic shape platelets. Conclusion: Results of this study showed that the mesenchymal surface of the AM is hemocompatible which could be a proper candidate for vascular tissue engineering. 1- Hoshi RA, Van Lith R, Jen MC, Allen JB, Lapidos KA, Ameer G. The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts. Biomaterials. 2013 34: 30-41. 2- Zhang WJ, Liu W, Cui L, Cao Y. Tissue engineering of blood vessel. J Cell Mol Med. 2007 11: 945-57. 3- Palumbo VD, Bruno A, Tomasello G, Damiano G, Lo Monte AI. Bioengineered vascular scaffolds: the state of the art. Int J Artif Organs. 2014 37: 503-12. 4- Suma H. Arterial grafts in coronary bypass surgery. Ann Thorac Cardiovasc Surg. 1999 5: 141-5. 5- Carnagey J, Hern-Anderson D, Ranieri J, Schmidt CE. 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Anticancer effects of human amniotic membrane and its epithelial cells. Med Hypotheses. 2014 82: 488-9. 12- Lee SB, Li DQ, Tan DT, Meller DC, Tseng SC. Suppression of TGF-beta signaling in both normal conjunctival fibroblasts and pterygial body fibroblasts by amniotic membrane. Curr Eye Res. 2000 20: 325-34. 13- Solomon A, Rosenblatt M, Monroy D, Ji Z, Pflugfelder SC, Tseng SC. Suppression of interleukin 1alpha and interleukin 1beta in human limbal epithelial cells cultured on the amniotic membrane stromal matrix. Br J Ophthalmol. 2001 85: 444-9. 14- Fernandes M, Sridhar MS, Sangwan VS, Rao GN. Amniotic membrane transplantation for ocular surface reconstruction. Cornea. 2005 24: 643-53. 15- Niknejad H, Deihim T, Solati-Hashjin M, Peirovi H. The effects of preservation procedures on amniotic membrane's ability to serve as a substrate for cultivation of endothelial cells. Cryobiology. 2011 63: 145-51. 16- Peirovi H, Rezvani N, Hajinasrollah M, Mohammadi SS, Niknejad H. Implantation of amniotic membrane as a vascular substitute in the external jugular vein of juvenile sheep. J Vasc Surg. 2012 56: 1098-104. 17- Niknejad H, Deihim T, Peirovi H, Abolghasemi H. Serum-free cryopreservation of human amniotic epithelial cells before and after isolation from their natural scaffold. Cryobiology. 2013 67: 56-63. 18- Ko TM, Lin JC, Cooper SL. Surface characterization and platelet adhesion studies of plasma-sulphonated polyethylene. Biomaterials. 1993 14: 657-64. 19- Goodman SL. Sheep, pig, and human platelet-material interactions with model cardiovascular biomaterials. J Biomed Mater Res. 1999 45: 240-50. 20- Motlagh D, Allen J, Hoshi R, Yang J, Lui K, Ameer G. Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering. J Biomed Mater Res A. 2007 82: 907-16. 21- Niknejad H, Khayat-Khoei M, Peirovi H, Abolghasemi H. Human amniotic epithelial cells induce apoptosis of cancer cells: a new anti-tumor therapeutic strategy. Cytotherapy. 2014 16: 33-40. 22- Niknejad H, Yazdanpanah G, Mirmasoumi M, Abolghasemi H, Peirovi H, Ahmadiani A. Inhibition of HSP90 could be possible mechanism for anti-cancer property of amniotic membrane. Med Hypotheses. 2013 81: 862-5. 23- Niknejed H, Yazdanpanah G, Khayat-khoei M. In vitro evaluation of the effects of amniotic membrane on viability and proliferation of cancer cells. J Zanjan Unive Med Sci. 2013 21: 13-21. 24- Lin WC, Liu TY, Yang MC. Hemocompatibility of polyacrylonitrile dialysis membrane immobilized with chitosan and heparin conjugate. Biomaterials. 2004 25: 1947-57. 25- Wennberg A, Hensten-Pettersen A. Sensitivity of erythrocytes from various species to in vitro hemolyzation. J Biomed Mater Res. 1981 15: 433-5. 26- Hao Y, Ma DH, Hwang DG, Kim WS, Zhang F. Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea. 2000 19: 348-52. 27- Murdoch AD, Dodge GR, Cohen I, Tuan RS, Iozzo RV. 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Suppression of corneal neovascularization by PEDF release from human amniotic membranes. Invest Ophthalmol Vis Sci. 2004 45: 1758-62. 32- Lee Y-M, Lee J-J, Shen M-Y, Hsiao G, Sheu J-R. Inhibitory mechanisms of activated matrix metalloproteinase-9 on platelet activation. Eur J Pharmacol. 2006 537: 52-8. 33- Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U. Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol Reprod. 2007 77: 577-88. 34- Oswald J, Boxberger S, Jorgensen B, et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells. 2004 22: 377-84. 35- Niknejad H, Paeini-Vayghan G, Tehrani FA, Khayat-Khoei M, Peirovi H. Side dependent effects of the human amnion on angiogenesis. Placenta. 2013 34: 340-5. 36- Mitchell SL, Niklason LE. Requirements for growing tissue-engineered vascular grafts. Cardiovasc Pathol. 2003 12: 59-64.

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