M.Sc. Defense

 | Post date: 2021/04/25 | 
Physiology Department
Mahsa Jazini Student
M.Sc Grade
Dr.Mahin Ganjkhani and Dr. Fatemeh Moradi Supervisors
The effect of social stress, enriched environment and exercise on neuroinflammasome NLRP3 pathway in the rat hippocampus pilocarpine model of epilepsy Title
21/10/2020 Date of thesis defense
Background:
Epilepsy is a chronic neurological disorder caused by overactive and simultaneous activity of neurons in the brain. In more than 30% of people with epilepsy, seizures cannot be controlled even with medication. On the other hand, social stress is one of the problems of today's societies that also play a role in epilepsy. Non-pharmacological approaches have been considered as ancillary strategies in the treatment of epilepsy. For instance, the use of enriched environment (EE) and exercise, that can be useful in addition to medication. Given that epilepsy can increase oxidative stress, it may be considered as an activator of inflammatory pathways. The aim of this study was to investigate the effect of social stress, enriched environment and exercise on oxidative stress indices and expression of inflammatory genes NLRP3, Caspase-1, IL18 and IL1β in male rats.
Method:
60 male rats were divided into 5 groups: control group, pilocarpine epilepsy group, epileptic group under social stress, epileptic group under enriched environment and epileptic group under exercise protocol. The animals were cared after the epilepsy model was developed, then epileptic rats were either housed in their respective social stress or in an enrichment environment conditions or an exercise protocol for 30 days.
Results:
At the end of the study, blood was taken from the animal to study the oxidative marker. In addition, brain hippocampal tissue was removed to evaluate the expression of NLRP3, Caspase-1, IL18 and IL1β genes
Conclusion:
Development of epilepsy model with pilocarpine led to increased oxidative marker and expression of inflammatory genes. Social stress also led to increased oxidative marker and expression of inflammatory genes. Enriched environment and exercise decreased oxidative markers, increased antioxidant markers and were able to reduce inflammatory genes.
Key words: Temporal lobe epilepsy, social stress, enriched environment, exercise, NLRP3 pathway
Abstract
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