Volume 29, Issue 135 (July & August 2021)                   J Adv Med Biomed Res 2021, 29(135): 189-196 | Back to browse issues page


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Hosseini S D, Rahimi M R, Abbaspoor M. Chronic Caffeine Ingestion Down-Regulates Liver and Visceral Adipose Tissue Inflammatory Gene Expression in High-Fat Diet-Induced Obesity. J Adv Med Biomed Res. 2021; 29 (135) :189-196
URL: http://zums.ac.ir/journal/article-1-6137-en.html
1- Dept. of Exercise Physiology, University of Kurdistan, Sanandaj, Iran , dara769@live.com
2- Dept. of Exercise Physiology, University of Kurdistan, Sanandaj, Iran
3- Dept. of Physical Education and Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Abstract:   (4495 Views)

Background and Objective: Beverages containing caffeine have an anti-obesity function. Reduction of visceral adipose tissue (VAT) inflammation is considered an important strategy to ameliorate obesity compilations such as insulin resistance. This study aimed to investigate the effect of 8-week caffeine supplementation on the messenger RNA (mRNA) expression of fetuin-A (FetA) in the liver and nuclear factor kappa B (Nf-κb) and toll-like receptor 4 (Tlr4) in the VAT of rats with a high-fat diet (HFD).
Methods: A total of 40 male Wistar rats were randomly divided into control, caffeine, HFD, and HFD+caffeine supplement groups. After 2 weeks of acclimatization, the rats were randomly fed with HFD (46% fat) and a normal diet (5% fat) for 8 weeks. The rats in the caffeine groups were gavaged with 6 mg of the caffeine solution per kg of body weight. FetA mRNA of the liver, Nf-κb, and Tlr4 mRNA of VAT were determined using real-time polymerase chain reaction (PCR).
Results: The results indicated that FetA mRNA expression and weight gain in HFD+caffeine were significantly reduced compared to the other groups. Nf-κb mRNA expression was significantly higher in the HFD group than in the caffeine groups. No statistically significant differences were found in Tlr4 mRNA expression between the groups.
Conclusion: These findings suggest that consuming caffeine can prevent HFD-induced liver and adipose tissue (AT) inflammation.

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These findings suggest that consuming caffeine can prevent HFD-induced liver and adipose tissue (AT) inflammation.


Type of Study: Original | Subject: Life science
Received: 2020/07/23 | Accepted: 2021/07/19 | Published: 2021/02/28

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